Generation of Transgenic Fluorescent Reporter Lines for Studying Hematopoietic Development in the Mouse.
Generation of Transgenic Fluorescent Reporter Lines for Studying Hematopoietic Development in the Mouse.
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用于研究小鼠造血发育的转基因荧光报告基因系的生成。
DOI：
10.1007/978-1-0716-1008-4_12
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Baron,MargaretH
中科院分区：
文献类型：
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作者：
Barminko,Jeffrey;Vacaru,AndreiM;Baron,MargaretH
小鼠和其他哺乳动物的造血发生在几个波，并从不同的解剖部位。转基因小鼠在造血系统的各个点表达荧光报告蛋白，从造血干细胞到更受限制的祖细胞，再到每种最终分化的细胞类型，为标记、跟踪和分离这些细胞提供了有价值的工具。在这一章中，我们将讨论设计转基因的一般考虑，调查可用的荧光探针，并描述用于确认和分析胚胎，胎儿和出生后/成年动物的造血组织中的转基因表达的方法。
Hematopoiesis in the mouse and other mammals occurs in several waves and arises from distinct anatomic sites. Transgenic mice expressing fluorescent reporter proteins at various points in the hematopoietic hierarchy, from hematopoietic stem cell to more restricted progenitors to each of the final differentiated cell types, have provided valuable tools for tagging, tracking, and isolating these cells. In this chapter, we discuss general considerations in designing a transgene, survey available fluorescent probes, and describe methods for confirming and analyzing transgene expression in the hematopoietic tissues of the embryo, fetus, and postnatal/adult animal.