Surfaces coated with protein layers:: a surface force and ESCA study
Surfaces coated with protein layers:: a surface force and ESCA study
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DOI：
10.1016/s0142-9612(97)00114-2
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发表时间：
1998-03-01
期刊：
影响因子：
14
通讯作者：
Fröberg, JC
中科院分区：
文献类型：
--
作者：
Blomberg, E;Claesson, PM;Fröberg, JC
在这篇综述文章中，我们讨论了蛋白质和表面之间相互作用的重要性的非特异性力的类型。其中一些力在理论上已经得到很好的理解，它们的性质也得到了详细的研究。其他类型的力的起源还不太清楚，甚至有争议，在这里我们将概括主要的实验发现。我们对界面力的认识大多来自于各种表面力技术的应用。本文介绍了两种这样的技术。典型的实验程序的概述和注意力被提请最常见的并发症，我们在我们的研究中遇到的。在这篇文章中，我们选择了目前的数据，说明了一些特定类型的力的存在，说明了一个特定的并发症，发生在评估实验结果时，或说明使用电子能谱化学分析（ESCA）结合表面力测量的优势。没有详细讨论系统，更详细的信息，我们将参考读者的原始文献。然而，我们提出了一个估计的量的小离子共吸附的ESCA和表面力装置（SFA）的结果的组合计算的蛋白质。这一估计是基于一个简单的电荷平衡，这需要一个知识的吸附量和扩散层电荷。吸附量是从ESCA测量使用的定量方法，利用已知的云母表面的化学组成。扩散层电荷是从用表面力技术测量的双层力的大小获得的。(C)1998爱思唯尔科技有限公司。保留所有权利。
In this review article we discuss the types of non-specific forces that are of importance for the interactions between proteins and surfaces. Some of these forces are well understood theoretically, and their properties investigated in detail. The origin of other types of forces is less well understood, or even controversial, and here we will recapitulate the main experimental findings. Most of our knowledge of interfacial forces stems from the use of various surface force techniques. Two such techniques are described in this article. The typical experimental procedure is outlined and attention is drawn to the most common complications that we have met during our studies. In this article we have chosen to present data that illustrate the presence of some particular type of force, that illustrate a particular complication that occurs when evaluating the experimental findings, or that illustrate the advantage of using electron spectroscopy for chemical analysis (ESCA) in combination with surface force measurements. No system is discussed in detail, and for more detailed information we will refer the reader to the original literature. However, we present an estimate of the amount of small ions co-adsorbed with the protein calculated from a combination of the ESCA and surface force apparatus (SFA) results. This estimate is based on a simple charge balance which requires a knowledge of the adsorbed amount and the diffuse layer charge. The adsorbed amount is obtained from ESCA measurements using a quantification method that utilizes the known chemical composition of the mica surface. The diffuse layer charge is obtained from the magnitude of the double-layer force measured with a surface force technique. (C) 1998 Elsevier Science Ltd. All rights reserved.