Immunohistochemical analysis of ageing human B and T cell populations reveals an age-related decline of CD8 T cells in spleen but not gut-associated lymphoid tissue (GALT)
Immunohistochemical analysis of ageing human B and T cell populations reveals an age-related decline of CD8 T cells in spleen but not gut-associated lymphoid tissue (GALT)
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DOI：
10.1016/s0047-6374(00)00106-8
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发表时间：
2000-05-18
影响因子：
5.3
通讯作者：
Dunn-Walters, DK
中科院分区：
文献类型：
--
作者：
Banerjee, M;Sanderson, JD;Dunn-Walters, DK
人们认为，免疫系统的衰老至少在一定程度上是许多与衰老相关的健康问题的原因。以前关于淋巴细胞成分变化的研究使用流式细胞术来研究外周血淋巴细胞(PBL)，即从啮齿动物组织中分离出来的细胞，并产生了相互矛盾的结果。我们用免疫组织化学的方法来确定人的脾和肠道组织中的B和T细胞是否受年龄的影响。测量B细胞滤泡生发中心、套区和边缘区的面积。计数T细胞区和B细胞滤泡中的CD4、CD8T细胞。我们观察到与年龄相关的CD8+T细胞在脾T细胞区的比例显著下降。这种下降在占据脾B细胞区的T细胞群中并不明显，在GALT中也不明显。T细胞区和B细胞区的T细胞群在CD4+细胞方面也有进一步的差异。这些发现突出了不同淋巴组织中淋巴细胞群体的差异，以及每个组织中不同的隔室，这可能对未来对衰老免疫系统的研究具有重要意义。(C)2000爱思唯尔爱尔兰科学有限公司。保留所有权利。
It is thought that senescence of the immune system is responsible, at least in part, for many health problems associated with ageing. Previous studies on changes in lymphocyte composition have used flow cytometry to study peripheral blood lymphocytes (PBL's), or cells isolated from rodent tissue, and have yielded conflicting results. We have used immunohistochemistry to determine whether the B and T cells in human tissue from spleen and gut are affected by age. Areas of germinal centre, mantle zone and marginal zone of B cell follicles were measured. In addition, CD4 and CD8 T cells in T cell areas and in B cell follicles were counted. We observed a striking age-related decrease in the proportion of CD8 + T cells in the T cell zones of the spleen. This decrease was not apparent in the T cell population that occupies splenic B cell areas, or in GALT. Further differences, in CD4 + cells, were seen between T cell populations in the T cell zones and those in B cell areas. These findings highlight differences between lymphocyte populations in different lymphoid tissues, and different compartments within each tissue, which may be of importance in future studies of the ageing immune system. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.