Determination of 18 O content of phosphate compounds--a novel method.

Determination of 18 O content of phosphate compounds--a novel method.
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磷酸盐化合物~(18)O含量的测定——一种新方法

DOI:
10.1016/0304-4165(72)90134-1
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发表时间:
1972
期刊:
Biochimica et biophysica acta
影响因子:
--
通讯作者:
J. Deutshi
J. Deutshi
中科院分区:
--
文献类型:
--
作者:
Jacob Bar;O. Ben;G. Rose;J. Deutshi

文献摘要

被引文献

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磷酸盐和含磷酸盐化合物的硅烷化反应为直接测定磷酸盐化合物中的18 O含量提供了一种合适的方法。描述了两个模型体系的硅烷化过程:H218 O水解ATP和18 O从[羧基-18O]乙酸转移到AMP。建议的程序的评价。
Silylation of phosphate and phosphate-containing compounds provides a suitable means for the direct measurement of the18O content of phosphate compounds. The silylation procedure is described for two model systems: ATP hydrolysis by H218O and18O transfer from [carboxy-18O] acetic acid to AMP. An evaluation of the suggested procedure is presented.