Whisker deafferentation and rodent whisking patterns: Behavioral evidence for a central pattern generator
Whisker deafferentation and rodent whisking patterns: Behavioral evidence for a central pattern generator
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DOI：
10.1523/jneurosci.21-14-05374.2001
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发表时间：
2001-07-15
影响因子：
5.3
通讯作者：
Zeigler, HP
中科院分区：
文献类型：
--
作者：
Gao, PH;Bermejo, R;Zeigler, HP
即使在没有明确刺激的情况下，大鼠也会发出有节奏的搅拌动作。由于其刻板的性质和感觉去神经和皮层消融后的持久性，搅拌运动已被假定为反映了中央模式发生器（CPG）的输出。然而，识别一个运动模式作为CPG的产品需要证据表明，它的产生，模式和协调是独立的感官输入。为了提供这样的证据，我们使用光电仪器来获得高分辨率记录的运动轨迹的个别胡须在大鼠的头部是固定的，以隔离他们的探索性搅拌从exafferent输入。无条件的搅拌模式进行了定量的生物特征分析的运动学，节奏，和协调的双边同源触须运动。单侧和双侧的眶下神经，支配胡须，然后进行切片，以阻止动物自己的搅拌运动产生的传入输入。单侧切片的神经没有影响搅拌运动学，但随后由一个显着的，但相对短暂的双边增加搅拌频率。然而，双边deafferentation，当在一个单一的阶段的过程中进行，不破坏的生成，图案，或双边协调的搅拌模式在大鼠。这些研究结果提供了强有力的行为证据搅拌CPG和讨论有关其可能的位置和属性。
Even in the absence of explicit stimulation, rats emit patterns of rhythmic whisking movements. Because of their stereotyped nature and their persistence after sensory denervation and cortical ablation, whisking movements have been assumed to reflect the output of a central pattern generator (CPG). However, identification of a movement pattern as the product of a CPG requires evidence that its generation, patterning, and coordination are independent of sensory input. To provide such evidence, we used optoelectronic instrumentation to obtain high-resolution records of the movement trajectories of individual whiskers in rats whose heads were fixed to isolate their exploratory whisking from exafferent inputs. Unconditioned whisking patterns were quantitatively characterized by a biometric analysis of the kinematics, rhythmicity, and coordination of bilaterally homologous vibrissa movements. Unilateral and bilateral sectioning of the infraorbital nerve, which innervates the whiskers, was then performed to block reafferent inputs generated by the animal's own whisking movements. Unilateral sectioning of the nerve has no effect on whisking kinematics but is followed by a significant but relatively transient bilateral increase in whisking frequency. However, bilateral deafferentation, when performed in a single-stage procedure, does not disrupt the generation, patterning, or bilateral coordination of whisking patterns in the rat. These findings provide strong behavioral evidence for a whisking CPG and are discussed in relation to its possible location and properties.