Molecular cloning of cDNAs of mouse peptidylarginine deiminase type I, type III and type IV, and the expression pattern of type I in mouse.
Molecular cloning of cDNAs of mouse peptidylarginine deiminase type I, type III and type IV, and the expression pattern of type I in mouse.
复制标题
小鼠肽基精氨酸脱亚胺酶I型、III型和IV型cDNA的分子克隆以及I型在小鼠中的表达模式。
DOI：
10.1046/j.1432-1327.1999.00083.x
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
H. Takahara
中科院分区：
文献类型：
--
作者：
A. A. Rus'd;Yasuko Ikejiri;Hiroyuki Ono;Toshihiro Yonekawa;M. Shiraiwa;Akira Kawada;H. Takahara
肽基精氨酸脱亚胺酶（Peptidylarginine deiminases，PAD）是一类翻译后酶，以钙离子依赖性方式催化蛋白质结合的精氨酸残基转化为瓜氨酸残基，广泛分布于脊椎动物的各种器官中。尽管据报道啮齿动物中存在四种PAD亚型（I型、II型、III型和IV型），但这些亚型在组织中共存的相对功能尚未得到探索。本研究通过筛选小鼠子宫cDNA文库，采用RACE方法克隆了编码小鼠PAD I型的全长cDNA。该cDNA由1989个碱基的开放阅读框、127个碱基的5 '非翻译区和1639个碱基的3'非翻译区组成，编码662个氨基酸（73，823 Da）。比较逆转录-PCR和Northern印迹分析检测PAD I型mRNA仅在表皮和子宫。给成年卵巢切除小鼠施用雌激素增加了子宫中PAD I型mRNA的含量，提供了其表达受性类固醇激素控制的证据。我们还通过RT-PCR和RACE方法克隆了小鼠PAD III型和IV型的全长cDNA。PAD III型的一级结构包含从1995个碱基的编码区推导出的664个氨基酸（75，098 Da），PAD IV型的一级结构由从2001个碱基的编码区推导出的666个氨基酸（74，475 Da）组成。4种PAD亚型的氨基酸序列同源性约为50%，其中3'端与5'端同源性较高。
Peptidylarginine deiminases (PADs), a group of post-translational enzymes, catalyze the conversion of protein-bound arginine residues to citrulline residues in a calcium ion-dependent manner and are widely distributed in various organs of vertebrates. Although the existence of four isoforms of PAD (types I, II, III, and IV) is reported in rodents, the relative functions of the isoforms with respect to their colocation in the tissues have yet to be explored. In this study, we cloned the full-length cDNA encoding mouse PAD type I by screening a uterine cDNA library and using the RACE method. This cDNA consists of an open reading frame of 1989 bases encoding 662 amino acids (73,823 Da), a 5'-untranslated region of 127 bases and a 3'-untranslated region of 1639 bases. Comparative reverse transcription-PCR and Northern-blot analyses detected PAD type I mRNA only in the epidermis and uterus. Administration of estrogen to adult ovariectomized mice increased the content of PAD type I mRNA in the uterus, providing evidence that its expression is under the control of the sex steroid hormone. We also cloned the full-length cDNAs of mouse PAD type III and type IV by the reverse transcription-PCR and RACE methods. The primary structure of PAD type III contains 664 amino acids (75,098 Da) deduced from the coding region of 1995 bases, and the primary structure of PAD type IV consists of 666 amino acids (74,475 Da) deduced from the coding region of 2001 bases. Comparison of the deduced amino acid sequences of all four isoforms of PAD showed about 50% identity with each other, the 3' regions being highly homologous compared with the 5' regions.
DOI：
10.1073/pnas.85.23.8998
发表时间：
1988-12-01
影响因子：
11.1
作者：
FROHMAN, MA;DUSH, MK;MARTIN, GR
通讯作者：
MARTIN, GR