"Peroxidatic" form of cytochrome oxidase as studied by X-ray absorption spectroscopy.
"Peroxidatic" form of cytochrome oxidase as studied by X-ray absorption spectroscopy.
复制标题
通过 X 射线吸收光谱研究的细胞色素氧化酶的“过氧化”形式。
DOI：
10.1016/s0006-3495(83)84309-4
复制
发表时间：
1983
影响因子：
3.4
通讯作者：
Ching,YC
中科院分区：
文献类型：
--
作者：
Chance,B;Kumar,C;Powers,L;Ching,YC
X-射线吸收光谱显示脉冲氧化酶类似于静息氧化酶，但缺乏活性位点的铁和铜之间的硫桥（Powers，L.，Y. Ching，B.吉斯和B Muhoberac，1982，Biophys. J.，37[2，Pt. 2]：403 a。【摘要】脉冲氧化酶活性位点血红素的第一壳配体和键长最清楚地适合辣根和酵母的铁过氧化物酶，并且脉冲氧化酶氰化物化合物类似于低自旋血红素蛋白氰化物化合物。结构结果与脉冲氧化酶的水或过氧形式一致，这也是通过光学研究观察到的。这些结构和化学数据与脉冲形式在循环过氧化物副反应中的作用一致，其中脉冲和脉冲过氧化物化合物充当过氧化物清除剂。非硫桥形式的细胞色素氧化酶的过氧化作用表明，在功能基础上的“氧化”或“脉冲”形式的细胞色素氧化酶的“过氧化”形式的重命名。
X-ray absorption spectroscopy shows pulsed oxidase to be similar to resting oxidase but to lack the sulfur bridge between iron and copper of active sites (Powers, L., Y. Ching, B. Chance, and B. Muhoberac, 1982, Biophys. J., 37[2, Pt. 2]: 403a. [Abstr.] ) The first shell ligands and bond lengths of the pulsed oxidase active site heme most clearly fit the ferric peroxidases from horseradish and yeast, and the pulsed oxidase cyanide compound resembles the low spin hemoprotein cyanide compounds. The structural results are consistent with an aquo or a peroxo form for pulsed oxidase as is also observed by optical studies. These structural and chemical data are consistent with a role for the pulsed forms in a cyclic peroxidatic side reaction in which the pulsed and pulsed peroxide compounds act as peroxide scavengers. The peroxidatic role of cytochrome oxidase in the nonsulfur bridged form suggests the renaming of the "oxygenated" or "pulsed" forms on a functional basis as "peroxidatic" forms of cytochrome oxidase.