Neuropeptide Y inhibits pertussis toxin-catalyzed ADP-ribosylation in bovine adrenal chromaffin cell membranes.
Neuropeptide Y inhibits pertussis toxin-catalyzed ADP-ribosylation in bovine adrenal chromaffin cell membranes.
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Neuropeptide Y 抑制牛肾上腺嗜铬细胞膜中百日咳毒素催化的 ADP-核糖基化。
DOI：
10.1016/0024-3205(93)90694-x
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发表时间：
1993
期刊：
影响因子：
6.1
通讯作者：
Hexum,TD
中科院分区：
文献类型：
--
作者：
Zhu,J;Hexum,TD
有证据表明，神经肽Y受体直接偶联到抑制G蛋白存在于培养的牛肾上腺染色质细胞膜。百日咳毒素催化41 kDa质膜蛋白的[32P] adp核糖基化。5 ' - guananylylimidodiphosphate以剂量依赖性的方式抑制该蛋白的[32P]ADP标记，而GTP则没有影响。用高浓度神经肽Y对质膜进行预孵育，然后短暂暴露于低浓度的5 ' -胍脲二磷酸显著；y抑制adp核糖基化的作用比单独使用5 ' -鸟苷二磷酸观察到的要好。这些结果表明，牛肾上腺染色质细胞中的神经肽Y受体直接偶联到41 kDa的PTX底物（可能是抑制性G蛋白的α亚基）。
Evidence is presented that the neuropeptide Y receptor is directly coupled to an inhibitory G protein existing in cultured bovine adrenal chromaffin cell membranes. Pertussis toxin catalyzes the [32P]ADP-ribosylation of a 41 kDa plasma membrane protein. 5′-Guanylylimidodiphosphate inhibited the [32P]ADP labelling of this protein in a dose-dependent manner whereas GTP had no effect. Preincubation of the plasma membranes with high concentrations of neuropeptide Y followed by a brief exposure to a low concentration of 5′-guanylylimidodiphosphate significant;y inhibited ADP-ribosylation beyond that observed with 5′-guanylimidodiphosphate alone. These result suggest that the neuropeptide Y receptor in bovine adrenal chromaffin cells is directly coupled to a 41 kDa PTX substrate (presumably the α subunit of an inhibitory G protein).