Modeling the Spatiotemporal Dynamics of Light and Heat Propagation for In Vivo Optogenetics.
Modeling the Spatiotemporal Dynamics of Light and Heat Propagation for In Vivo Optogenetics.
复制标题
DOI：
10.1016/j.celrep.2015.06.036
复制
发表时间：
2015-07-21
期刊：
影响因子：
8.8
通讯作者：
Gordon JA
中科院分区：
文献类型：
--
作者：
Stujenske JM;Spellman T;Gordon JA
尽管光遗传学在体内的应用越来越多，但直接暴露于光对脑组织的影响尚未得到充分研究。特别值得关注的是，长时间的光刺激可能会导致发热。我们证明，高强度的光，通过光纤传递，是能够提高局部放电率，即使在没有视蛋白表达。因此，预测光遗传学刺激期间任何温度升高的严重程度和空间范围是相当重要的。在这里，我们描述了一个逼真的模型，模拟光遗传学实验过程中的光和热传播。我们通过比较预测和测量的体内温度变化来验证该模型。我们通过比较各种光波长和纤维尺寸的预测，以及减少体内神经靶点热效应的测试方法，进一步证明了该模型的实用性。
Despite the increasing use of optogenetics in vivo, the effects of direct light exposure to brain tissue are understudied. Of particular concern is the potential for heat induced by prolonged optical stimulation. We demonstrate that high intensity light, delivered through an optical fiber, is capable of elevating firing rate locally, even in the absence of opsin expression. Predicting the severity and spatial extent of any temperature increase during optogenetic stimulation is therefore of considerable importance. Here we describe a realistic model that simulates light and heat propagation during optogenetic experiments. We validated the model by comparing predicted and measured temperature changes in vivo. We further demonstrate the utility of this model by comparing predictions for various wavelengths of light and fiber sizes, as well as testing methods for reducing heat effects on neural targets in vivo.