Detection and infectivity potential of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) environmental contamination in isolation units and quarantine facilities
Detection and infectivity potential of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) environmental contamination in isolation units and quarantine facilities
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DOI：
10.1016/j.cmi.2020.09.004
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发表时间：
2020-12-01
影响因子：
14.2
通讯作者：
Weiss, Shay
中科院分区：
文献类型：
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作者：
Ben-Shmuel, Amir;Brosh-Nissimov, Tal;Weiss, Shay
目标：环境表面被认为可能是导致 COVID-19 传播的因素。本研究评估了两个医院隔离病房和一家检疫酒店中严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 污染表面和物体的传染性。方法：在受控实验室条件下测试非多孔表面上的 SARS-CoV-2 病毒稳定性和传染性。在两个 COVID-19 隔离单位和一家检疫酒店进行了表面和空气采样。通过RT-PCR检测病毒RNA，并通过VERO E6 CPE测试评估感染性。结果：在实验室控制条件下，SARS-CoV-2在环境温度下第4天逐渐完全丧失感染性，表面病毒活力的衰减率与温度升高直接相关。在两家医院隔离病房和一家无症状和极轻症 COVID-19 患者隔离酒店的有症状 COVID-19 患者周围环境的 29/55 个表面样本（52.7%）和 16/42 个表面样本（38%）中检测到病毒 RNA。组织培养测定中，三个地点的表面和空气样本 (0/97) 均未发现含有传染性滴度的 SARS-Cov-2。结论：尽管 SARS-CoV-2 在实验室控制条件下的存活时间较长，但无生命表面的不可培养病毒污染可能表明间接污染物传播的可行性较低。 (C) 2020 作者。由爱思唯尔有限公司代表欧洲临床微生物学和传染病学会出版。
Objectives: Environmental surfaces have been suggested as likely contributors in the transmission of COVID-19. This study assessed the infectivity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contaminating surfaces and objects in two hospital isolation units and a quarantine hotel.Methods: SARS-CoV-2 virus stability and infectivity on non-porous surfaces was tested under controlled laboratory conditions. Surface and air sampling were conducted at two COVID-19 isolation units and in a quarantine hotel. Viral RNA was detected by RT-PCR and infectivity was assessed by VERO E6 CPE test.Results: In laboratory-controlled conditions, SARS-CoV-2 gradually lost its infectivity completely by day 4 at ambient temperature, and the decay rate of viral viability on surfaces directly correlated with increase in temperature. Viral RNA was detected in 29/55 surface samples (52.7%) and 16/42 surface samples (38%) from the surroundings of symptomatic COVID-19 patients in isolation units of two hospitals and in a quarantine hotel for asymptomatic and very mild COVID-19 patients. None of the surface and air samples from the three sites (0/97) were found to contain infectious titres of SARS-Cov-2 on tissue culture assay.Conclusions: Despite prolonged viability of SARS-CoV-2 under laboratory-controlled conditions, uncultivable viral contamination of inanimate surfaces might suggest low feasibility for indirect fomite transmission. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases.