ODD-PAIRED - A ZINC-FINGER PAIR RULE PROTEIN REQUIRED FOR THE TIMELY ACTIVATION OF ENGRAILED AND WINGLESS IN DROSOPHILA EMBRYOS
ODD-PAIRED - A ZINC-FINGER PAIR RULE PROTEIN REQUIRED FOR THE TIMELY ACTIVATION OF ENGRAILED AND WINGLESS IN DROSOPHILA EMBRYOS
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DOI：
10.1101/gad.8.1.105
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发表时间：
1994-01-01
影响因子：
10.5
通讯作者：
DINARDO, S
中科院分区：
文献类型：
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作者：
BENEDYK, MJ;MULLEN, JR;DINARDO, S
成对规则基因，奇数配对（opa），是果蝇胚胎的准分节细分所必需的。除了其先前定义的作用在奇数parasegments的wingless（wg）的激活，我们发现，opa是必要的及时激活wg在其余parasegments和及时激活englued（en）在所有parasegments。opa编码锌指蛋白，其指与果蝇节极性基因ci（D）、人胶质母细胞瘤基因GLI和秀丽隐杆线虫性别决定基因tra-1的指同源。先前的工作表明opa活性对于交替旁片段的建立是必不可少的，这表明opa的表达或活性将像其他配对规则基因一样受到空间限制。相反，opa mRNA和蛋白质在所有节原基中发现。因此，opa不以空间受限的方式起作用以建立en和wg表达的位置。相反，opa必须与其他空间限制蛋白质合作，以实现果蝇胚胎的适当细分。
The pair-rule gene, odd-paired (opa), is essential for parasegmental subdivision of the Drosophila embryo. In addition to its previously defined role in the activation of wingless (wg) in odd parasegments, we find that opa is required for the timely activation of wg in the remaining parasegments and for the timely activation of engrailed (en) in all parasegments. opa encodes a zinc finger protein with fingers homologous to those of the Drosophila segment polarity gene ci(D), the human glioblastoma gene GLI and the Caenorhabditis elegans sex determination gene tra-1. Previous work showed that opa activity was essential for the establishment of alternate parasegments, suggesting opa expression or activity would be spatially restricted like other pair-rule genes. Instead, opa mRNA and protein are found throughout all segment primordia. Thus, opa does not act in a spatially restricted manner to establish the position of en and wg expression. Rather, opa must cooperate with other spatially restricted proteins to achieve proper subdivision of the Drosophila embryo.