MicroRNA-223 expression in neutrophils in the early phase of secondary damage after spinal cord injury
MicroRNA-223 expression in neutrophils in the early phase of secondary damage after spinal cord injury
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DOI：
10.1016/j.neulet.2011.01.068
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发表时间：
2011-04-01
影响因子：
2.5
通讯作者：
Ochi, Mitsuo
中科院分区：
文献类型：
--
作者：
Izumi, Bunichiro;Nakasa, Tomoyuki;Ochi, Mitsuo
microRNA（miR）是一类抑制靶基因翻译的短链非编码RNA，在基因调控中起着重要作用。尽管如此突出，很少有报道提到脊髓损伤（SCI）后miR的表达。之前，我们报道了小鼠SCI后miR-223的表达。本研究的目的是揭示miR-223在损伤脊髓中的分布，并鉴定表达miR-223的细胞。定量聚合酶链反应分析显示，高表达的miR-223在12小时后SO。原位杂交和免疫组化双重染色显示miR-223信号与Gr-1阳性中性粒细胞融合。我们的数据表明，miR-223可能在SCI后的早期阶段调节中性粒细胞。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
MicroRNA (miR)s are short non-coding RNAs that suppress the translation of target genes, and play an important role in gene regulation. Despite this prominence, there are few reports that refer to the expression of miRs after spinal cord injury (SCI). Previously, we reported on miR-223 expression after SCI in mice. The purpose of this study is to reveal the distribution of miR-223 and identify the cells that express miR-223 in the injured spinal cord. Quantitative polymerase chain reaction analysis revealed high expression of miR-223 at 12 h after SO. Double staining of in situ hybridization and immunohistochemistry showed that the signals of miR-223 merged with Gr-1 positive neutrophils. Our data indicate that miR-223 might regulate neutrophils in the early phase after SCI. (C) 2011 Elsevier Ireland Ltd. All rights reserved.