Classification and Characterization of Hemocytes in Styela clava.
Classification and Characterization of Hemocytes in Styela clava.
复制标题
海鞘中血细胞的分类和表征。
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
E. Cooper
中科院分区：
文献类型：
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作者：
T. Sawada;J. Zhang;E. Cooper
有活力的细胞分为四组：嗜酸性粒细胞、嗜碱性粒细胞、透明细胞和淋巴细胞样细胞。嗜酸性粒细胞，活跃性变形虫，有大的折射颗粒，染色呈中性红色。嗜碱性粒细胞不染中性红色，形成成对或三胞胎。透明细胞通常含有吞噬体，具有电子密集的小泡，只有在电子显微镜下才能识别。在光镜下可见造血细胞具有特征性的大核仁。嗜酸性粒细胞和透明细胞在体外积极摄取酵母颗粒。这种分类通过将电子显微镜、光学显微镜和固定血细胞进行比较，简化了以前的分类。通过简单的方法可以清楚地识别出活的被囊细胞。我们提供了清晰和更准确的描述，这将减少在体内和体外免疫防御反应中分配血细胞功能的争议。
Viable hemocytes of the common tunicate Styela clava are classified into four groups designated as eosinophilic granulocytes, basophilic granulocytes, hyaline cells and lymphocyte-like cells. Eosinophilic granulocytes, actively amoeboid, have large refractive granules that stain with neutral red. Basophilic granulocytes do not stain with neutral red and formed couplets or triplets. Hyaline cells, which often contain phagosomes, have electron-dense small vesicles recognizable only by electron microscopy. Hemoblasts have a characteristic large nucleolus which is visible by light microscopy. Eosinophilic granulocytes and hyaline cells actively ingest yeast particles in vitro. This classification simplifies former ones by correlating electron microscopy, with light microscopy, and viable with fixed hemocytes. Clearly viable tunicate hemocytes can be identified by simple methods. We have provided clear and more accurate descriptions which will lessen the controversy often associated with assigning hemocyte functions in immunodefense responses both in vivo and in vitro.