Radiation Sensitivities in the Terminal Stages of Megakaryocytic Maturation and Platelet Production
Radiation Sensitivities in the Terminal Stages of Megakaryocytic Maturation and Platelet Production
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DOI：
10.1667/rr1519.1
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发表时间：
2009-09-01
影响因子：
3.4
通讯作者：
Kashiwakura, Ikuo
中科院分区：
文献类型：
--
作者：
Monzen, Satoru;Osuda, Koichi;Kashiwakura, Ikuo
这些研究检查了X射线和白细胞介素3（IL-3），这是一个有效的细胞因子的生成巨核细胞从X射线照射的造血干/祖细胞，对人类巨核细胞和血小板生成的终端过程的影响。通过在无血清液体培养物中培养正常人外周血中的CD 34（+）细胞诱导成熟巨核细胞，所述无血清液体培养物用血小板生成素刺激。实验包含以下组：对照培养物与未照射的细胞孵育15天;培养物在第7天或第11天用IL-3处理，培养物在第7天或第11天用2戈伊照射，培养物在X射线照射后立即用IL-3处理。未辐照的对照培养物从第7天开始产生巨核细胞，巨核细胞和血小板生成在第12-13天达到高峰。当在第7天进行X射线照射时，巨核细胞和血小板数量均显著减少，而当在第11天进行X射线照射时，未观察到对这些数量的显著影响。IL-3对巨核细胞成熟和血小板生成的终末阶段既没有保护作用，也没有促进作用。结果表明，成熟巨核细胞具有放射敏感性，但随着巨核细胞成熟的末期，尤其是进入前血小板形成期的巨核细胞，其放射敏感性降低。(C)2009年，辐射研究学会
These studies examined the effects of X radiation and interleukin 3 (IL-3), which is an effective cytokine for the generation of megakaryocytopoiesis from X-irradiated hematopoietic stem/progenitor cells, on the terminal process of human megakaryocytopoiesis and thrombopoiesis. Mature megakaryocytes were induced by culturing CD34(+) cells from normal human peripheral blood in a serum-free liquid culture stimulated with thrombopoietin. The experiments contained the following groups: control cultures with nonirradiated cells incubated for 15 days; cultures treated with IL-3 on day 7 or day 11, cultures irradiated with 2 Gy on day 7 or day 11, and cultures treated with IL-3 immediately after X irradiation. The nonirradiated control cultures produced megakaryocytes from day 7, and both the megakaryocyte and platelet generation reached a peak on day 12-13. When X irradiation was performed on day 7, both the megakaryocyte and platelet numbers decreased remarkably, while no significant effect was observed on those numbers when cultures were X-irradiated on day 11. IL-3 showed neither protective nor promoting effects on the terminal stages of megakaryocytic maturation and platelet production. The results demonstrated that mature megakaryocytes are radiosensitive but that the radiosensitivity decreased with the terminal stages of megakaryocytic maturation, especially for the megakaryocytes entering into proplatelet formation. (C) 2009 by Radiation Research Society