Advances in Photosynthesis Research
Advances in Photosynthesis Research
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光合作用研究进展
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
Kazuo Shibata
中科院分区：
文献类型：
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作者：
Kazuo Shibata
通过提取物的体内光谱和色谱 (HPLC) 研究了在不同温度下放置在黑暗中且不摇动的烟草细胞（烟草变种“Samsun”）悬浮培养物中叶绿素的分解。随着细胞孵育时间和/或温度的增加，Soret谱带移动至较短波长，表明细胞中脱镁叶绿素化，但此后该移动反转至较长波长。衰老细胞提取物的洗脱色谱图仅在不同色素的相对量上发生变化：叶绿素 a、脱镁叶绿素 a、脱镁叶绿酸 a、极性更强的脱镁叶绿酸 a（称为“X”），有时是脱镁叶绿素 a 和其他两个小峰。根据与真实样品的光谱（UV-Vis、MS）、色谱（TLC、HPLC）和化学（转化为甲酯）相似性得出的结论，“X”被假定为 13-羟基脱镁叶绿酸 a。
The breakdown of chlorophyll in suspension cultures of tobacco cells (Nicotiana tabacum var. "Samsun") placed in the dark without shaking at different temperatures was investigated by in-vivo spectroscopy and chromatography (HPLC) of the extracts. With increasing time and/ or temperatures of incubation of the cells the Soret band shifts to shorter wavelengths indicating pheophytinization in the cells, but afterwards this shift is reversed to longer wavelengths. The pattern of the elution chromatogram of the extracts from senescing cells changes only in the relative amount of the different pigments: chlorophyll a, pheophytin a, pheophorbide a, a more polar pheophorbide a called "X", sometimes chlorophyllide a and two other minor peaks. "X" is assumed to be 13-hydroxypheophorbide a as concluded from the spectroscopic (UV-Vis, MS), chromatographic (TLC, HPLC) and chemical (conversion to the methylester) similarities with an authentic sample.