TEM analysis of nanoparticle dispersions with application towards the quantification of in vitro cellular uptake
TEM analysis of nanoparticle dispersions with application towards the quantification of in vitro cellular uptake
复制标题
纳米颗粒分散体的 TEM 分析及其在体外细胞摄取定量中的应用
DOI：
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
A. Brown
中科院分区：
文献类型：
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作者：
N. Hondow;P. Wang;R. Brydson;M. Holton;P. Rees;H. Summers;A. Brown
由于在样品制备过程中发生的干燥效应，纳米颗粒在溶液中分散体的TEM分析以前主要限于测量初级颗粒尺寸。我们表明，制备TEM的解决方案，通过浸渍冷冻试样网格涂有印迹溶液是一个敏感的和潜在的代表性的路线来测量纳米粒子的分散。我们已经开始对更常用的动态光散射方法的技术进行基准测试，并表明TEM路线是潜在的更敏感的路线，以计数分散体中的单个纳米颗粒，也包含纳米颗粒团聚体。准确测量纳米粒子在生物溶液中的分散度是纳米粒子在医学中应用的关键步骤。
TEM analysis of nanoparticle dispersions in solution has previously been limited, in the main, to the measurement of primary particle size due to the drying effects that occur during sample preparation. We show that solutions prepared for TEM by plunge freezing specimen grids coated with a blotted solution is a sensitive and potentially representative route to measuring a nanoparticle dispersion. We have started to benchmark the technique against the more commonly used dynamic light scattering method and show that the TEM route is potentially more sensitive route to counting individual nanoparticles in a dispersion that also contains nanoparticle agglomerates. Accurate measurement of nanoparticle dispersion in biological solutions could be a key step in the application of nanoparticles in medicine.
影响因子：
10.8
作者：
Shi J;Votruba AR;Farokhzad OC;Langer R
通讯作者：
Langer R