IFT52 plays an essential role in sensory cilia formation and neuronal sensory function in Drosophila
IFT52 plays an essential role in sensory cilia formation and neuronal sensory function in Drosophila
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IFT52 在果蝇感觉纤毛形成和神经元感觉功能中发挥重要作用
DOI：
10.1111/1744-7917.13140
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发表时间：
2022-12-22
期刊：
影响因子：
4
通讯作者：
Wei, Qing
中科院分区：
文献类型：
--
作者：
Hou, Ya-Nan;Zhang, Ying-Ying;Wei, Qing
纤毛是以微管为基础的毛状细胞器，参与感觉功能或运动，在生殖、器官发育和感觉等许多生理过程中发挥关键作用。在昆虫中，纤毛仅限于某些感觉神经元和精子，对化学和机械感觉以及生育能力都很重要。虽然纤毛组装的机制已经取得了很大的进展，但昆虫纤毛的形成仍然知之甚少，甚至在昆虫模式生物果蝇中也是如此。鞭毛内转运(IFT)是一种纤毛特异性复合体，沿纤毛轴丝双向运输蛋白质货物，对大多数纤毛是必不可少的。在这里，我们研究了IFT-B的核心成分IFT52在果蝇纤毛/鞭毛形成中的作用。我们发现果蝇IFT52沿感觉神经元纤毛分布，是感觉神经纤毛形成所必需的。Ift52缺失会导致纤毛相关感觉行为的严重缺陷。值得注意的是，在果蝇精母细胞纤毛和精子鞭毛中没有检测到IFT52。因此，ift52突变体可以产生具有正常运动能力的精子，这支持了ift在果蝇精子鞭毛形成中的重要作用。总之，IFT52是一种保守的蛋白质，对昆虫的感觉纤毛形成和感觉神经元功能是必不可少的。
Cilia are microtubule-based, hair-like organelles involved in sensory function or motility, playing critical roles in many physiological processes such as reproduction, organ development, and sensory perception. In insects, cilia are restricted to certain sensory neurons and sperms, being important for chemical and mechanical sensing, and fertility. Although great progress has been made regarding the mechanism of cilia assembly, the formation of insect cilia remains poorly understand, even in the insect model organism Drosophila. Intraflagellar transport (IFT) is a cilia-specific complex that traffics protein cargos bidirectionally along the ciliary axoneme and is essential for most cilia. Here we investigated the role of IFT52, a core component of IFT-B, in cilia/flagellar formation in Drosophila. We show that Drosophila IFT52 is distributed along the sensory neuronal cilia, and is essential for sensory cilia formation. Deletion of Ift52 results in severe defects in cilia-related sensory behaviors. It should be noted that IFT52 is not detected in spermatocyte cilia or sperm flagella of Drosophila. Accordingly, ift52 mutants can produce sperms with normal motility, supporting a dispensable role of IFT in Drosophila sperm flagella formation. Altogether, IFT52 is a conserved protein essential for sensory cilia formation and sensory neuronal function in insects.