Relationship between Genetic Variants of Mitochondrial DNA and Growth Traits in Hanwoo Cattle
Relationship between Genetic Variants of Mitochondrial DNA and Growth Traits in Hanwoo Cattle
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韩牛牛线粒体DNA遗传变异与生长性状的关系
DOI：
10.5713/ajas.2005.301
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
H. H. Sung
中科院分区：
文献类型：
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作者：
G. Jeon;H. Chung;J. G. Choi;M. S. Lee;Chang;J. J. Park;J. Ha;Hye;H. H. Sung
利用限制性内切酶检测了韩虎mtDNA细胞色素氧化酶亚基I、II和III区遗传变异。根据牛mtDNA序列设计引物，共使用Mt4、Mt5、Mt6、Mt7、Mt8和Mt9 6组引物。共使用了20个限制性内切酶，其中Hinf I、Pvu II、Rsa I、Eco RI、Bgl II和Msp I 6个限制性内切酶存在遗传多态性。细胞色素氧化酶亚基复合物区域遗传变异与Mt9 Pvu II、Mt6 Bgl II和Mt8 Rsa I显著相关（p<0.05）， WT15和WT18与Mt9 Pvu II、Mt6 Bgl II和Mt8 Rsa I显著相关。WT9 （p=0.01）和WT12 （p=0.02）在Mt9-Pvu II和Mt6-Bgl II片段上也分别观察到显著的相关性。这些结果表明，细胞色素氧化酶亚基复合物区域mtDNA的遗传变异可能是改善动物生长和体重性状的候选片段。(Asian-Aust。j .似的。科学》2005。Vol 18, No. 3: 301-307)
Genetic variants of Hanwoo mtDNA in the region of cytochrome oxidase subunit I, II and III complex were detected using restriction enzymes. PCR primers were designed based on the bovine mtDNA sequence, and 6 primer sets (Mt4, Mt5, Mt6, Mt7, Mt8 and Mt9) were used. A total of 20 restriction enzymes were used, and 6 restriction enzymes, which were Hinf I, Pvu II, Rsa I, Eco RI, Bgl II, and Msp I, showed genetic polymorphisms. Significant associations between genetic variants and weight traits were observed at WT15 (p<0.05) and WT18 (p<0.01) with Pvu II for Mt9, Bgl II for Mt6 and Rsa I for Mt8 segments in the region of cytochrome oxidase subunit complex. Significant associations were also observed at Mt9-Pvu II and Mt6-Bgl II segments for WT9 (p=0.01), WT12 (p=0.02), respectively. These results suggest that genetic variants of mtDNA in the region of cytochrome oxidase subunit complex may be candidate segments for improvement of animal growth as weight traits. (Asian-Aust. J. Anim. Sci. 2005. Vol 18, No. 3 : 301-307)