Study of urinary 6β-hydroxycortisol/cortisol ratio in spot urine sample as a biomarker of 3A4 enzyme activity in healthy and epileptic subjects of Egyptian population
Study of urinary 6β-hydroxycortisol/cortisol ratio in spot urine sample as a biomarker of 3A4 enzyme activity in healthy and epileptic subjects of Egyptian population
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DOI：
10.1016/j.phrs.2005.02.014
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发表时间：
2005-06-01
影响因子：
9.3
通讯作者：
Siest, JP
中科院分区：
文献类型：
--
作者：
El Desoky, ES;Mohamed, HO;Siest, JP
采用ELIZA试剂盒（Stabiligen）对埃及男女健康成人（n = 65，年龄16-48岁）于上午8点至下午12点采集的尿样中尿6 β -羟基皮质醇/皮质醇（6 β - ohc /FQ）的比值进行了研究。尿6 β - ohc /FC比值频率分布广泛，男性高于女性。两性均未见双峰现象。另一组成人癫痫患者（n = 16）研究慢性卡马西平抗癫痫药物给药对尿样尿6 - ohc /FC比值的影响。卡马西平对CYP3A4的诱导作用，癫痫患者6 β - ohcifc比值较对照组显著升高（p = 0.01）。总之，埃及人尿6 β - ohc /FC比值的频率分布呈现两性二态性。此外，测定尿6 β - ohc /FC比值为监测卡马西平抗癫痫药物给药期间CYP3A4酶的诱导提供了一种简单无创的方法。(c) 2005 Elsevier Ltd版权所有。
The ratio of urinary 6 beta-hydroxycortisol/cortisol (6 beta-OHC/FQ in morning spot urine samples collected from 8:00 a.m. to 12:00 p.m. was studied using ELIZA kits (Stabiligen) in a group of healthy adult Egyptians (control group) of both sex (n = 65, age range: 16-48 years). The frequency distribution of urinary 6 beta-OHC/FC ratio was widely distributed among subjects with higher values in males in comparison to females. No bimodality in either sex was observed. Another group of adult epileptic patients (n = 16) was studied for the influence of chronic carbamazepine antiepileptic drug administration on urinary 6 beta-OHC/FC ratio in spot urine samples. The induction property of carbamazepine on CYP3A4 was observed through significant increase (p = 0.01) in 6 beta-OHCIFC ratio among epileptic patients in comparison with control subjects. In conclusion, the frequency distribution of urinary 6 beta-OHC/FC ratio among Egyptians shows sexual dimorphism. Also, measurement of urinary 6 beta-OHC/FC ratio provides a simple non-invasive method to monitor CYP3A4 enzyme induction during administration of carbamazepine antiepileptic drug. (c) 2005 Elsevier Ltd. All rights reserved.