Histomorphometry of Feline Chronic Kidney Disease and Correlation With Markers of Renal Dysfunction
Histomorphometry of Feline Chronic Kidney Disease and Correlation With Markers of Renal Dysfunction
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DOI：
10.1177/0300985812453176
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发表时间：
2013-01-01
影响因子：
2.4
通讯作者：
Elliott, J.
中科院分区：
文献类型：
--
作者：
Chakrabarti, S.;Syme, H. M.;Elliott, J.
慢性肾脏疾病在老年猫中很常见，但大多数病例有非特异性肾脏病变，很少有研究将这些病变与肾功能不全的临床病理标志物相关联。本研究的目的是确定与慢性肾脏病猫的肾功能最相关的病变和疾病进展的可能介质。在1992年至2010年期间，通过2个第一意见实践招募了猫。当批准尸检时，将肾组织保存在福尔马林中。由对所有临床病理学数据不知情的病理学家评价切片。对肾小球硬化、间质炎症和纤维化的严重程度进行半定量评分。使用图像分析测量肾小球体积;记录陈旧肾小球的百分比。评估切片的增生性小动脉硬化和肾小管矿化。本研究纳入了80只猫的肾脏，其血浆生化数据来自生命的最后2个月。多变量线性回归（P <0.05）用于评估病变与接近死亡时获得的临床病理学数据的相关性。间质纤维化是与氮质血症、高磷血症和贫血严重程度相关性最好的病变。蛋白尿与间质纤维化和肾小球肥大相关，而较高的时间平均收缩压与肾小球硬化和增生性小动脉硬化相关。
Chronic kidney disease is common in geriatric cats, but most cases have nonspecific renal lesions, and few studies have correlated these lesions with clinicopathological markers of renal dysfunction. The aim of this study was to identify the lesions best correlated with renal function and likely mediators of disease progression in cats with chronic kidney disease. Cats were recruited through 2 first-opinion practices between 1992 and 2010. When postmortem examinations were authorized, renal tissues were preserved in formalin. Sections were evaluated by a pathologist masked to all clinicopathological data. They were scored semiquantitatively for the severity of glomerulosclerosis, interstitial inflammation, and fibrosis. Glomerular volume was measured using image analysis; the percentage of glomeruli that were obsolescent was recorded. Sections were assessed for hyperplastic arteriolosclerosis and tubular mineralization. Kidneys from 80 cats with plasma biochemical data from the last 2 months of life were included in the study. Multivariable linear regression (P < .05) was used to assess the association of lesions with clinicopathological data obtained close to death. Interstitial fibrosis was the lesion best correlated with the severity of azotemia, hyperphosphatemia, and anemia. Proteinuria was associated with interstitial fibrosis and glomerular hypertrophy, whereas higher time-averaged systolic blood pressure was associated with glomerulosclerosis and hyperplastic arteriolosclerosis.