Synergistic action of MLL, a TRX protein with template activating factor‐I, a histone chaperone
Synergistic action of MLL, a TRX protein with template activating factor‐I, a histone chaperone
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DOI：
10.1016/j.febslet.2004.12.064
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发表时间：
2005-01
期刊：
影响因子：
3.5
通讯作者：
T. Shimoyama;Kohsuke Kato;Mary Miyaji-Yamaguchi;K. Nagata
中科院分区：
文献类型：
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作者：
T. Shimoyama;Kohsuke Kato;Mary Miyaji-Yamaguchi;K. Nagata
MLL参与了基因活性维持的过程。结果表明，MLL(MLLN)的氨基末端区域与TAF-Iβ/SET相互作用。在本研究中，利用酵母双杂交实验，我们发现TAF-Iβ的酸性区域是其与MLLN结合所必需的。GST-MLLN的下拉分析表明，Taf-Iβ和组蛋白与GST-MLLN相互作用。MLLN和TAF-Iβ协同上调HOXA9的转录水平，并在含有HOXA9启动子区域的染色质中免疫共沉淀。这些结果表明，TAF-Iβ在MLL介导的转录和可能的染色质维持中起重要作用。
MLL is involved in the process of gene activity maintenance. It is shown that the amino-terminal region of MLL (MLLN) interacts with TAF-Iβ/SET. In this study, using yeast two-hybrid assays, we have found that the acidic region of TAF-Iβ is essential for its binding to MLLN. Pull-down assays using GST-MLLN demonstrated that TAF-Iβ and histones interact with GST-MLLN. MLLN and TAF-Iβ synergistically upregulated the transcription level of Hoxa9 and co-immunoprecipitated in chromatin containing the Hoxa9 promoter region. These results suggest that TAF-Iβ plays an important role in MLL-mediated transcription and possibly chromatin maintenance.