Heterochromatin and tri-methylated lysine 20 of histone H4 in animals
Heterochromatin and tri-methylated lysine 20 of histone H4 in animals
复制标题
DOI：
10.1242/jcs.01238
复制
发表时间：
2004-05-15
影响因子：
4
通讯作者：
Singh, PB
中科院分区：
文献类型：
--
作者：
Kourmouli, N;Jeppesen, P;Singh, PB
组蛋白H4上的三甲基化赖氨酸20(Me(3)K20H4)是小鼠间期和中期细胞构成异染色质的标志。异染色质以Me标记(3)K20H4在细胞周期的S期复制较晚。血清饥饿增加了在构成异染色质处显示高水平Me(3)K20H4的细胞的数量。Me(3)K20H4也存在于精子发生过程中大多数减数分裂染色体的着丝粒异染色质和假常染色体区域，以及一些端粒。它不存在于XY-Body上。在小鼠胚胎发生过程中，母体原核含有Me(3)K20H4，父体原核不含Me(3)K20H4。在果蝇多线染色体Me(3)上，K20H4以“点状模式”存在于许多染色体带上，包括染色中心。在球虫中，它存在于兼性异染色化的父系染色体上。我们还提供了Me(3)K20H4依赖于H3特异性SUV(3)9组蛋白甲基转移酶活性的证据，表明组蛋白H3和H4之间可能存在表观遗传串扰。
Tri-methylated lysine 20 on histone H4 (Me(3)K20H4) is a marker of constitutive heterochromatin in murine interphase and metaphase cells. Heterochromatin marked by Me(3)K20H4 replicates late during S phase of the cell cycle. Serum starvation increases the number of cells that exhibit high levels of Me(3)K20H4 at constitutive heterochromatin. Me(3)K20H4 is also present at the centromeric heterochromatin of most meiotic chromosomes during spermatogenesis and at the pseudoautosomal region, as well as at some telomeres. It is not present on the XY-body. During murine embryogenesis the maternal pronucleus contains Me(3)K20H4; Me(3)K20H4 is absent from the paternal pronucleus. On Drosophila polytene chromosomes Me(3)K20H4 is present in a 'punctate pattern' at many chromosomal bands, including the chromocenter. In coccids it is present on the facultatively heterochromatinised paternal chromosome set. We also present evidence that Me(3)K20H4 is dependent upon H3-specific Suv(3)9 histone methyltransferase activity, suggesting that there may be 'epigenetic cross-talk' between histones H3 and H4.