Growth characteristics of tubercle bacilli and certain other mycobacteria in HeLa cells.
Growth characteristics of tubercle bacilli and certain other mycobacteria in HeLa cells.
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DOI：
10.1084/jem.105.1.39
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发表时间：
1957-01-01
期刊：
影响因子：
--
通讯作者：
SHEPARD CC
中科院分区：
文献类型：
--
作者：
SHEPARD CC
通过利用HeLa细胞暴露在含有精选马血清的组织培养液中后吞噬作用的增加，有可能将所有研究的分枝杆菌物种导入细胞，其中许多分枝杆菌继续在细胞中生长。在几天内，完全毒力的结核杆菌菌株填满了大部分细胞质，并形成了其他菌株所没有的特征索状物。据说毒力较弱的菌株R1Rv、BCG、H37Ra和R1Ra的生长速度较慢，且具有特征模式。它们在HeLa细胞中的增殖率与它们对小鼠和豚鼠的致病性有很好的相关性。六株抗异烟肼菌株的生长速度具有完全毒力菌株的特征。在“快速生长”的物种中，草分枝杆菌和污垢分枝杆菌没有表现出细胞生长的证据，尽管偶然分枝杆菌有。一些在37℃以下的细菌培养基上最适温度的菌株，如巴氏分枝杆菌、海洋分枝杆菌和白斑分枝杆菌，在HeLa细胞中生长迅速，特别是在31至35℃的温度下。
By making use of the increased phagocytosis which follows the exposure of HeLa cells to tissue culture media containing selected horse sera, it was possible to introduce all of the mycobacterial species studied into the cells, where many of them proceeded to grow. Fully virulent strains of tubercle bacilli filled much of the cytoplasm in a few days and formed characteristic cords not seen with other strains. The strains said to be less virulent, R1Rv, BCG, H37Ra, and R1Ra, grew less rapidly and in characteristic patterns. Their rates of multiplication in HeLa cells were in the order named and correlated well with their reported pathogenicity for mice and guinea pigs. Six INH-resistant strains grew at rates characteristic of fully virulent strains. Among the "rapidly growing" species, M. phlei and M. smegmatis did not show evidence of growth in the cells, although M. fortuitum did. Some strains with optimal temperatures on bacteriological media below 37°C, M. balnei, M. marinum, and M. platypoecilus, grew rapidly in HeLa cells, especially at temperatures of 31 to 35°C. The growth patterns of the bacilli in HeLa cells appear sufficiently specific to be useful in differentiation among the mycobacteria.