The endothelial pocket. A new structure in fenestrated endothelia.
The endothelial pocket. A new structure in fenestrated endothelia.
复制标题
内皮袋。
DOI：
10.1007/bf00212526
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发表时间：
1986
影响因子：
3.6
通讯作者：
Palade,GE
中科院分区：
文献类型：
--
作者：
Milici,AJ;Peters,KR;Palade,GE
通过对肾小管周毛细血管的联合透射和扫描电子显微镜研究，发现了一种新的内皮细胞结构，称为内皮囊。对这些和其他有窗口的毛细血管进行的透射电子显微镜观察表明，每个口袋都由一层稀疏的有窗口的内皮细胞折叠组成，在内皮细胞表面其余部分上方的管腔中突出200 nm。在这个管腔褶皱之下，有一个空间，然后是另一层紧靠基板的有窗内皮细胞。测得小鼠肾皮质、肠粘膜和胰腺外分泌部毛细血管内皮袋的线密度分别为0.067、0.017和0.007袋·μm-1。阳离子铁蛋白修饰这些毛细血管床内皮细胞管腔表面的阴离子部位显示，未标记和标记的横隔膜都是成簇的。在这类标本中，大多数内皮袋上的管腔横隔膜没有扫描或透射电子显微镜可检测到的阳离子铁蛋白结合部位。在这一点上，考虑到它们的一般形态，内皮囊似乎是简单的跨内皮细胞通道的多个版本。
A new endothelial cell structure, named the endothelial pocket, has been found by combined transmission and scanning electron microscopic studies of renal peritubular capillaries. Transmission EM observations made on these and other fenestrated capillaries demonstrated that each pocket consists of an attenuated fold of fenestrated endothelium that projects 200 nm into the lumen above the rest of the endothelial surface. Beneath this luminal fold, there is a space and then another layer of fenestrated endothelium which abuts the basal lamina. The linear density of endothelial pockets was measured in the capillaries of the kidney cortex, intestinal mucosa and exocrine pancreas in mice and determined to be 0.067, 0.017 and 0.007 pockets·μm-1respectively. Cationic ferritin decoration of the anionic sites on the luminal surface of the endothelium in these capillary beds revealed that both unlabelled and labelled diaphragms are clustered. In such specimens, the majority of the luminal diaphragms on endothelial pockets did not have cationic ferritin binding sites detectable by either scanning or transmission EM. On this account as well as on account of their general morphology, endothelial pockets appear to be multifold versions of the simple transendothelial channel.