Microbial communities associated with the invasive Hawaiian sponge Mycale armata
Microbial communities associated with the invasive Hawaiian sponge Mycale armata
复制标题
与入侵夏威夷海绵 Mycale armata 相关的微生物群落
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
Emilie Lefait
中科院分区：
文献类型：
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作者：
Guangyi Wang;Sang‐Hwal Yoon;Emilie Lefait
微生物共生体对其宿主生态至关重要，但入侵海洋物种的微生物群落在很大程度上仍未被探索。克隆文库和变性梯度凝胶电泳分析揭示了入侵海绵Mycale armata中不同的微生物群落类型。系统型与八个门相关：变形菌门、放线菌门、拟杆菌门、蓝细菌门、酸细菌门、绿球藻门、泉古菌门和厚壁菌门，其中α变形菌序列占优势（>58%）。在M中鉴定出3个细菌系统型群（BPG 1--仅与海绵序列相关; BPG 2--与海绵和其它海洋生物相关; BPG 3--潜在的新细菌类型）。武装。BPG 2-B类群的操作分类单位（OTU）是杜鹃花科（Rhododocellaceae）两个克隆文库的优势序列。armata的序列，但仅构成非侵入性海绵Dysidea sp.的一小部分序列。armata与其参考序列的同源性较低，因此是潜在的新类型。我们的结果表明，M. armata具有海绵特有的细菌类型和来自其他海洋生物的细菌类型。
Microbial symbionts are fundamentally important to their host ecology, but microbial communities of invasive marine species remain largely unexplored. Clone libraries and Denaturing gradient gel electrophoresis analyses revealed diverse microbial phylotypes in the invasive marine sponge Mycale armata. Phylotypes were related to eight phyla: Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Acidobacteria, Chloroflexi, Crenarchaeota and Firmicutes, with predominant alphaproteobacterial sequences (>58%). Three Bacterial Phylotype Groups (BPG1––associated only with sequence from marine sponges; BPG2––associated with sponges and other marine organisms and BPG3––potential new phylotypes) were identified in M. armata. The operational taxonomic units (OTU) of cluster BPG2-B, belonging to Rhodobacteraceae, are dominant sequences of two clone libraries of M. armata, but constitute only a small fraction of sequences from the non-invasive sponge Dysidea sp. Six OTUs from M. armata were potential new phylotypes because of their low sequence identity with their reference sequences. Our results suggest that M. armata harbors both sponge-specific phylotypes and bacterial phylotypes from other marine organisms.