Green tea polyphenols modify gut-microbiota dependent metabolisms of energy, bile constituents and micronutrients in female Sprague-Dawley rats.
Green tea polyphenols modify gut-microbiota dependent metabolisms of energy, bile constituents and micronutrients in female Sprague-Dawley rats.
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DOI：
10.1016/j.jnutbio.2018.07.018
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发表时间：
2018-11
期刊：
影响因子：
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通讯作者：
Jun Zhou;L. Tang;Chwan-Li Shen;Jia-Sheng Wang
中科院分区：
文献类型：
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作者：
Jun Zhou;L. Tang;Chwan-Li Shen;Jia-Sheng Wang
我们最近的宏基因组学分析发现，绿色茶多酚（GTP）对大鼠肠道微生物群结构和能量转换相关基因直系同源物具有显着的修饰作用。这些基因组变化如何进一步影响宿主健康仍不清楚。在这项工作中，在GTP处理的大鼠中研究了肠道微生物群依赖性代谢物的变化。对6组雌性SD大鼠（n=12/组）给予含0%、0.5%和1.5%GTP（wt/vol）的饮用水。在3月龄和6月龄时收集其肠道内容物，并通过高效液相色谱（HPLC）和气相色谱（GC）-质谱（MS）进行分析。基于GC-MS的代谢物组学分析捕获了2668个特征，从NIST碎片数据库识别的前200个差异特征中排除了57种代谢物。使用标准校准方法定量一组关键代谢物。与对照组相比，GTP处理组中升高的成分包括烟酸（8.61倍），3-苯基乳酸（2.20倍），半乳糖（3.13倍），甘露糖（2.05倍），十五烷酸（2.15倍），乳酸（2.70倍）和脯氨酸（2.15倍）;还原组分包括胆固醇（0.29倍）、胆酸（0.62倍）、脱氧胆酸（0.41倍）、海藻糖（0.14倍）、葡萄糖（0.46倍）、果糖（0.12倍）和丙氨酸（0.61倍）。这些结果与先前通过宏基因组学分析发现的肠道微生物组的基因组改变一致。这些代谢物的变化表明，减少热量的碳水化合物，维生素生产的升高，胆汁成分的减少，并在GTP处理的动物的氨基酸的代谢模式的修改。肠道微生物群相关代谢的变化可能是GTP抗肥胖功能的主要贡献者。
Our recent metagenomics analysis has uncovered remarkable modifying effects of green tea polyphenols (GTP) on gut-microbiota community structure and energy conversion related gene orthologs in rats. How these genomic changes could further influence host health is still unclear. In this work, the alterations of gut-microbiota dependent metabolites were studied in the GTP-treated rats. Six groups of female SD rats (n=12/group) were administered drinking water containing 0%, 0.5%, and 1.5% GTP (wt/vol). Their gut contents were collected at 3 and 6 months and were analyzedviahigh performance liquid chromatography (HPLC) and gas chromatography (GC)-mass spectrometry (MS). GC–MS based metabolomics analysis captured 2668 feature, and 57 metabolites were imputatively from top 200 differential features identifiedviaNIST fragmentation database. A group of key metabolites were quantitated using standard calibration methods. Compared with control, the elevated components in the GTP-treated groups include niacin (8.61-fold), 3-phenyllactic acid (2.20-fold), galactose (3.13-fold), mannose (2.05-fold), pentadecanoic acid (2.15-fold), lactic acid (2.70-fold), and proline (2.15-fold); the reduced components include cholesterol (0.29-fold), cholic acid (0.62-fold), deoxycholic acid (0.41-fold), trehalose (0.14-fold), glucose (0.46-fold), fructose (0.12-fold), and alanine (0.61-fold). These results were in line with the genomic alterations of gut-microbiome previously discovered by metagenomics analysis. The alterations of these metabolites suggested the reduction of calorific carbohydrates, elevation of vitamin production, decreases of bile constituents, and modified metabolic pattern of amino acids in the GTP-treated animals. Changes in gut-microbiota associated metabolism may be a major contributor to the anti-obesity function of GTP.