Cell-specific production and antimicrobial activity of naphthoquinones in roots of Lithospermum erythrorhizon
Cell-specific production and antimicrobial activity of naphthoquinones in roots of Lithospermum erythrorhizon
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DOI：
10.1104/pp.119.2.417
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发表时间：
1999-02-01
期刊：
影响因子：
7.4
通讯作者：
Flores, HE
中科院分区：
文献类型：
--
作者：
Brigham, LA;Michaels, PJ;Flores, HE
紫草素的有色萘醌衍生物在紫草根的特定时间和特定细胞中产生。正常的色素发展仅限于根毛和根边缘细胞在毛状根生长的“非诱导”介质，而诱导额外的色素生产的非生物（硫酸铜）或生物（真菌诱导子）因素增加的总色素的量，改变的比例产生的衍生物，并启动生产的色素从头表皮细胞。当测试这些化合物对土壤传播的细菌和真菌的生物活性时，记录了广泛的敏感性。乙酰紫草素和β-羟基异戊酰紫草素是发根农杆菌转化的“毛状根”培养物和温室生长的植物根中最丰富的两种衍生物，是测试的七种化合物中最具生物活性的。病原真菌立枯丝核菌，腐霉aphanidermatum，和Nectria hematocca的菌丝诱导局部色素生产与根接触后。挑战R。Solani粗诱导子使紫草素衍生物产量增加30倍。我们已经研究了这套相关的，差异产生的，差异活性化合物的调节，以了解它们在根际细胞水平上在植物防御中的作用。
Pigmented naphthoquinone derivatives of shikonin are produced at specific times and in specific cells of Lithospermum erythrorhizon roots. Normal pigment development is limited to root hairs and root border cells in hairy roots grown on "noninducing" medium, whereas induction of additional pigment production by abiotic (CuSO4) or biotic (fungal elicitor) factors increases the amount of total pigment, changes the ratios of derivatives produced, and initiates production of pigment de novo in epidermal cells. When the biological activity of these compounds was tested against soil-borne bacteria and fungi, a wide range of sensitivity was recorded. Acetyl-shikonin and beta-hydroxyisovaleryl-shikonin, the two most abundant derivatives in both Agrobacterium rhizogenes-transformed "hairy-root" cultures and greenhouse-grown plant roots, were the most biologically active of the seven compounds tested. Hyphae of the pathogenic fungi Rhizoctonia solani, Pythium aphanidermatum, and Nectria hematococca induced localized pigment production upon contact with the roots. Challenge by R. solani crude elicitor increased shikonin derivative production 30-fold. We have studied the regulation of this suite of related, differentially produced, differentially active compounds to understand their role(s) in plant defense at the cellular level in the rhizosphere.