Combined effects of carbonate alkalinity and pH on survival, growth and haemocyte parameters of the Venus clam Cyclina sinensis
Combined effects of carbonate alkalinity and pH on survival, growth and haemocyte parameters of the Venus clam Cyclina sinensis
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DOI：
10.1016/j.fsi.2013.05.006
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发表时间：
2013-08-01
影响因子：
4.7
通讯作者：
Wang, Hui
中科院分区：
文献类型：
--
作者：
Lin, Tingting;Lai, Qifang;Wang, Hui
碳酸盐碱度（CA）和pH值被认为是两个重要的压力因素，决定了水生动物的反应突然转移到盐碱水。为了评价盐碱水环境中青蛤（Cyclina sinensis）的养殖生产潜力，连续10 d每天监测CA（2.5（对照）、10.0、20.0和40.0 meq/l）和pH（8.0（对照）、8.5、9.0和9.5）对存活率的综合影响。在相同的水温（20 ℃）和盐度（15 ppm）条件下，分别测定了3、6、12和24 d的总血细胞数（THC）、吞噬率（PR）和血细胞死亡率（HM）。结果表明，CA处理组的成活率显著高于对照组（P <0.05），而其他处理组的成活率差异显著，尤其是在第3和第6天（P < 0.05），且随着CA或pH值的增加，成活率差异显著，但在第12天恢复到对照组水平。总之，在本研究中，观察到CA和pH之间的强相互作用。此外，可以确定的是，文蛤C sinensis可以承受CA 20.0结合pH 9.0的压力，虽然个体生长缓慢，可能需要大约12天才能恢复到非应激条件。(C)2013爱思唯尔有限公司保留所有权利。
Carbonate alkalinity (CA) and pH are considered to be two important stress factors that determine the response of aquatic animals to sudden transfers into saline-alkaline water. To evaluate the potential for aquaculture production of Venus clams (Cyclina sinensis) farmed in saline-alkaline water, the combined effects of CA (2.5 (control), 10.0, 20.0 and 40.0 meq/l) and pH (8.0 (control), 8.5, 9.0 and 9.5) on survival rate was monitored every day for 10 days. Length gain rate (LGR) and weight gain rate (WGR) were also monitored for two months, and total haemocyte count (THC), phagocytic rate (PR) and haemocyte mortality (HM) were measured for 3, 6, 12 and 24 days under the same water temperature (20 degrees C) and salinity (15 parts per thousand) conditions. The results showed that survival rates in treatments of CA 0.05), however, in other treatments, they presented significant differences, especially on day 3 and 6 (P < 0.05), most notably with increases in CA or pH, but returned to control levels on day 12. In conclusion, in this study, a strong interaction between CA and pH was observed. Additionally, it was ascertained that the Venus clam C sinensis can withstand the stress of CA 20.0 combined pH 9.0, although individuals grows slowly and may take approximately 12 days to recover to the unstressed condition. (C) 2013 Elsevier Ltd. All rights reserved.