Olfactory Neurons Expressing Closely Linked and Homologous Odorant Receptor Genes Tend to Project Their Axons to Neighboring Glomeruli on the Olfactory Bulb
Olfactory Neurons Expressing Closely Linked and Homologous Odorant Receptor Genes Tend to Project Their Axons to Neighboring Glomeruli on the Olfactory Bulb
复制标题
表达紧密相连且同源的气味受体基因的嗅觉神经元倾向于将其轴突投射到嗅球上邻近的肾小球
作者：
A. Tsuboi;Sei;Nika Yamazaki;Hiroaki Kasai;Hisae Asai;Madoka Komatsu;Shou Serizawa;T. Ishii;Yoichi Matsuda;F. Nagawa;H. Sakano
我们鉴定了两个独立的气味受体(OR)基因簇，以研究表达紧密连锁和同源OR基因的嗅神经元是如何将轴突投射到嗅球的。小鼠的OR基因MOR28、MOR10和MOR83在氨基酸序列上有75%-95%的相似性，并紧密连锁在14号染色体上。原位杂交表明，这三个基因在嗅觉上皮最背外侧和最内侧的同一区域表达，很少在单个神经元中同时表达。此外，我们还发现，表达MOR28、MOR10或MOR83的嗅神经元将它们的轴突投射到嗅球内侧和外侧非常接近但不同的肾小球亚群。2号染色体上A16和MOR18的另一个小鼠OR基因簇也得到了类似的结果，氨基酸序列有91%的相似性。这些结果可能表明了一种有趣的可能性，即在一个簇中表达同源OR基因的嗅觉神经元倾向于将它们的轴突会聚到肾小球近端但不同的亚群。这些研究路线将阐明嗅觉神经元向嗅球的地形性投射的分子基础。
We have characterized two separate odorant receptor (OR) gene clusters to examine how olfactory neurons expressing closely linked and homologous OR genes project their axons to the olfactory bulb. Murine OR genes, MOR28, MOR10, and MOR83, share 75–95% similarities in the amino acid sequences and are tightly linked on chromosome 14. In situ hybridization has demonstrated that the three genes are expressed in the same zone, at the most dorsolateral and ventromedial portions of the olfactory epithelium, and are rarely expressed simultaneously in individual neurons. Furthermore, we have found that olfactory neurons expressing MOR28, MOR10, or MOR83 project their axons to very close but distinct subsets of glomeruli on the medial and lateral sides of the olfactory bulb. Similar results have been obtained with another murine OR gene cluster for A16 and MOR18 on chromosome 2, sharing 91% similarity in the amino acid sequences. These results may indicate an intriguing possibility that olfactory neurons expressing homologous OR genes within a cluster tend to converge their axons to proximal but distinct subsets of glomeruli. These lines of study will shed light on the molecular basis of topographical projection of olfactory neurons to the olfactory bulb.
DOI：
10.1073/pnas.90.8.3329
发表时间：
1993-04-15
影响因子：
11.1
作者：
GUTHRIE, KM;ANDERSON, AJ;GALL, C
通讯作者：
GALL, C