Alterations of Group I mGluRs and BDNF Associated with Behavioral Abnormity in Prenatally Stressed Offspring Rats
Alterations of Group I mGluRs and BDNF Associated with Behavioral Abnormity in Prenatally Stressed Offspring Rats
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I 组 mGluRs 和 BDNF 的改变与产前应激子代大鼠的行为异常相关
DOI：
10.1007/s11064-015-1565-6
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发表时间：
2015-05-01
影响因子：
4.4
通讯作者：
Zhu, Zhongliang
中科院分区：
文献类型：
--
作者：
Jia, Ning;Li, Qinghong;Zhu, Zhongliang
大量证据表明，产前应激与后代的异常行为密切相关，但其机制尚不清楚。本研究旨在观察产前应激对仔鼠行为、代谢型谷氨酸受体1/5（mGluR 1/5）和脑源性神经营养因子（BDNF）的影响。对1月龄仔鼠进行强迫游泳试验（FST）和旷场试验（OFT）。测量后代大鼠不同脑区mGluR 1、mGluR 5和BDNF mRNA的表达水平。结果表明，晚期产前应激（LPS）组大鼠在FST中的不动时间较对照组明显延长，尤以雌性大鼠为甚。同样，在OFT中，中期产前应激（MPS）和LPS组的大鼠均表现出焦虑样行为，尤其是LPS组的雄性大鼠。LPS组大鼠海马和前额叶皮质mGluR 1蛋白表达增加，MPS组大鼠前额叶皮质mGluR 1蛋白表达增加。同时，LPS组大鼠海马和前额叶皮层mGluR 5蛋白表达增强。MPS组和LPS组雌性大鼠纹状体mGluR 5蛋白表达均增加，LPS组仅雄性大鼠纹状体mGluR 5蛋白表达增加。LPS组大鼠海马、前额叶皮质及雌性大鼠纹状体中BDNF mRNA表达水平明显降低。这些结果表明mGluR 1、mGluR 5和BDNF mRNA的改变可能与产前应激子代大鼠的抑郁样和焦虑样行为有关。
Substantial evidence reveals that prenatal stress is closely linked with abnormal behavior in offspring, but the mechanism remains unclear. In this study, our aim was to observe the alterations of behaviors, metabotropic glutamate receptor-1/5 (mGluR1/5) and brain-derived neurotrophic factor (BDNF) in various brain regions of prenatally stressed offspring rats. The forced swimming test (FST) and the open field test (OFT) were carried on 1-month-old offspring rats. The expression levels of mGluR1, mGluR5, and BDNF mRNA were measured in various brain regions of the offspring rats. Our results showed that the immobile time in the FST was significantly increased in the late prenatal stress (LPS) group compared with that in the control group, especially in the female rats. Similarly, in the OFT, the rats in both the mid prenatal stress (MPS) and LPS groups demonstrated anxiety-like behavior, especially the male rats in the LPS group. The expression of mGluR1 protein was increased in the hippocampus and prefrontal cortex of rats from the LPS group, as well as in the prefrontal cortex of rats from the MPS group. Meanwhile, the expression of mGluR5 protein was facilitated in the hippocampus and prefrontal cortex of rats in the LPS group. The expression of mGluR5 protein was increased in the striatum of the female rats in both MPS and LPS groups, and only in the male rats from the LPS group. In addition, the reduced BDNF mRNA level was detected in the hippocampus and prefrontal cortex in the LPS rats, and in the striatum of the female rats in LPS group. These results indicate that alterations of the mGluR1, mGluR5 and BDNF mRNA may contribute to the depression-like and anxiety-like behaviors of prenatally stressed offspring rats.