Metallosomes: artificial cell membranes with ultrathin metallic surfaces derived from cationic cerasomes through electroless plating
Metallosomes: artificial cell membranes with ultrathin metallic surfaces derived from cationic cerasomes through electroless plating
复制标题
金属体：通过化学镀从阳离子陶瓷体中获得的具有超薄金属表面的人造细胞膜
DOI：
--
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
T. Imori
中科院分区：
文献类型：
--
作者：
Feng Gu;Mineo Hashizume;Shusuke Okada;Y. Sasaki;J. Kikuchi;T. Imori
具有金属表面的新型脂质体膜，即所谓的金属体，是通过无电镀阳离子神经酰胺体制备的，该神经酰胺体是最近开发的有机-无机囊泡纳米杂化物。通过静电作用将PdCl 4 2-离子结合到阳离子膜表面，然后将该前体催化剂还原为Pd（0），最后使用合适的镀液在神经酰胺脂质体表面沉积一层金属，从而进行神经酰胺脂质体的化学镀.虽然可以通过阳离子神经酰胺脂质体的无电镀镍来制备涂覆有Ni包被层的金属脂质体，但是不可能从在类似的电镀条件下用阳离子肽脂质形成的脂质体膜中获得相应的Ni包被囊泡，因此反映了这些囊泡的形态稳定性的差异。使用各种物理测量如扫描和透射电子显微镜、能量色散X射线光谱、电子能量损失光谱和电子断层扫描进行Ni-金属体的表征。通过改变镀覆时间，可以在纳米尺度上控制镍层厚度。通过差示扫描量热法观察到Ni-金属脂质体的凝胶到液晶相转变行为，表明金属脂质体保持其脂质双层膜。由相应的神经酰胺脂质体制备了直径为50-5000 nm的各种尺寸的镍金属脂质体。通过化学镀Ni/Au置换镀Ni-金属体，也成功地获得了具有Au层的金属体。
Novel liposomal membranes having a metallic surface, so-called metallosomes, were prepared by electroless plating of cationic cerasomes, recently developed as organic-inorganic vesicular nanohybrids. The electroless plating of cerasomes was performed by firstly binding PdCl 4 2- ions onto the cationic membrane surface through electrostatic interactions, then subsequently reducing this precursor catalyst to Pd (0), and finally, depositing a layer of metal onto the cerasome surface using an appropriate plating bath. While metallosomes coated with an ultrathin Ni layer could be prepared by electroless Ni plating of cationic cerasomes, it was not possible to derive the corresponding Ni-coated vesicles from the liposomal membranes formed with cationic peptide lipids under similar plating conditions, thus reflecting the difference in the morphological stability of these vesicles. The characterization of the Ni-metallosomes was performed using various physical measurements such as scanning and transmission electron microscopies, energy-dispersive X-ray spectroscopy, electron energy-loss spectroscopy, and electron tomography. The Ni layer thickness was controllable on the nanometer scale by changing the plating time. The gel to liquid-crystalline phase transition behavior of the Ni-metallosomes was observed by differential scanning calorimetry, indicating that the metallosomes maintain their lipid bilayer membranes. Ni-metallosomes with various sizes were prepared from the corresponding cerasomes in a diameter range of 50-5000 nm. Metallosomes with an Au layer were also successfully obtained by electroless Ni/Au substitution plating of Ni-metallosomes.
DOI：
10.1073/pnas.93.21.11443
发表时间：
1996-10-15
影响因子：
11.1
作者：
Moscho, A;Orwar, O;Zare, RN
通讯作者：
Zare, RN