EPIGENETIC CONTROL OF AN ENDOGENOUS GENE FAMILY IS REVEALED BY A NOVEL BLUE FLUORESCENT MUTANT OF ARABIDOPSIS
EPIGENETIC CONTROL OF AN ENDOGENOUS GENE FAMILY IS REVEALED BY A NOVEL BLUE FLUORESCENT MUTANT OF ARABIDOPSIS
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DOI：
10.1016/0092-8674(95)90185-x
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发表时间：
1995-12-01
期刊：
影响因子：
64.5
通讯作者：
FINK, GR
中科院分区：
文献类型：
--
作者：
BENDER, J;FINK, GR
拟南芥Wassilewskija株含有4个编码色氨酸酶磷酸核糖基氨基苯甲酸异构酶（phosphoribosyllanthranilate isomerase，派）的基因，位于3个非连锁位点，这4个派基因在其DNA同源区域发生甲基化，当缺失2个串联排列的基因时，派拷贝数减少（MePAI 1-PAI 4），具有荧光的突变体，Escherichan缺陷表型结果，因为剩下的两个甲基化派基因这两个甲基化的基因可以通过减数分裂遗传，即使当它们在与具有未甲基化的派基因的菌株的杂交中彼此分离时，然而，由甲基化派基因赋予的突变体表型是不稳定的，并且突变体植物偶尔产生回复突变体细胞区段和后代。回复突变株系显示PAI 2和PAI 3的甲基化协同降低，这意味着这种低甲基化在整个基因组中以协同的方式起作用，而不是在单个位点起作用。
The Wassilewskija strain of Arabidopsis has four genes encoding the tryptophan enzyme phosphoribosylanthranilate isomerase (PAI) located at three unlinked sites, These four PAI genes are methylated over their regions of DNA homology, When PAI copy number is reduced by deletion of two tandemly arrayed genes (MePAI1-PAI4), a mutant with fluorescent, tryptophan-deficient phenotypes results, because the two remaining methylated PAI genes (MePAI2 and MePAI3) supply insufficient PAI activity, These two methylated genes can be inherited through meiosis, even when they are segregated away from each other in crosses to a strain with unmethylated PAI genes, However, the mutant phenotypes conferred by the methylated PAI genes are unstable, and mutant plants yield occasional revertant somatic sectors and progeny. Revertant lines display coordinately reduced methylation of both PAI2 and PAI3, implying that this hypomethylation acts in a concerted manner across the genome rather than at individual sites.