The Knockdown of Maternal Glucocorticoid Receptor mRNA Alters Embryo Development in Zebrafish
The Knockdown of Maternal Glucocorticoid Receptor mRNA Alters Embryo Development in Zebrafish
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DOI：
10.1002/dvdy.22586
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发表时间：
2011-04-01
影响因子：
2.5
通讯作者：
Dalla Valle, L.
中科院分区：
文献类型：
--
作者：
Pikulkaew, S.;Benato, F.;Dalla Valle, L.
在斑马鱼中，排卵的卵母细胞含有母体皮质醇和糖皮质激素受体（gr）的mRNA，其作为颗粒结构遍布卵质。在0.2 hpf时，该转录本被重新定位在胚盘区并在卵裂球之间分配。在6- 8hpf时，它被合子转录本取代。我们使用吗啉代阻断母体和合子gr转录本的翻译，并使用错剪接吗啉代阻断转录后合子转录本。只有敲低翻译产生的细胞凋亡和随后的颅面和尾部畸形与严重畸形的神经，血管和内脏器官的胚胎和5-DPF幼虫的增加。这些缺陷被拯救与鳟鱼gr 2 mRNA。微阵列分析显示，114和37个高表达的成绩单上调和下调，分别由母亲Gr蛋白缺乏5-hpf胚胎。这些结果表明，母体gr转录本和蛋白质参与了斑马鱼发育的母体编程。Developmental Dynamics 240：874-889，2011. (C)2011 Wiley-Liss，Inc.
In zebrafish, ovulated oocytes contain both maternal cortisol and the mRNA for the glucocorticoid receptor (gr), which is spread as granular structures throughout the ooplasm. At 0.2 hpf, this transcript is relocated in the blastodisc area and partitioned among blastomeres. At 6-8 hpf, it is replaced by zygotic transcript. We used morpholinos to block translation of both maternal and zygotic gr transcripts, and a missplicing morpholino to block post-transcriptionally the zygotic transcript alone. Only knockdown of translation produced an increase of apoptosis and subsequent craniofacial and caudal deformities with severe malformations of neural, vascular, and visceral organs in embryos and 5-dpf larvae. Such defects were rescued with trout gr2 mRNA. Microarray analysis revealed that 114 and 37 highly expressed transcripts were up-and down-regulated, respectively, by maternal Gr protein deficiency in 5-hpf embryos. These results indicate that the maternal gr transcript and protein participate in the maternal programming of zebrafish development. Developmental Dynamics 240:874-889, 2011. (C) 2011 Wiley-Liss, Inc.