Elongation factor SII-dependent transcription by RNA polymerase II through a sequence-specific DNA-binding protein.
Elongation factor SII-dependent transcription by RNA polymerase II through a sequence-specific DNA-binding protein.
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RNA 聚合酶 II 通过序列特异性 DNA 结合蛋白进行延伸因子 SII 依赖性转录。
DOI：
10.1073/pnas.90.5.1917
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发表时间：
1993
影响因子：
11.1
通讯作者：
MoteJr,J
中科院分区：
文献类型：
--
作者：
Reines,D;MoteJr,J
在真核生物中，遗传物质包含在一种称为染色质的卷曲的、被蛋白质覆盖的结构中。RNA聚合酶必须识别特定的核蛋白组合，并与数千个碱基对的潜在DNA双链保持接触。来自大肠杆菌的模板结合的乳胶操纵子抑制物在体外和体内抑制RNA聚合酶II[Kuhn，A.，Bartsch，I.&Grummt，I.(1990)Nature(London)344,559-562；Deuschele，U，Hipskind，R.A.&Bujard，H.(1990)Science 248,480-483]。我们发现，在重组的转录系统中，延伸因子SII使RNA聚合酶II能够高效地通过这种阻断。Lac抑制子-抑制延伸复合体显示SII激活的转录切割反应，这一活性与先前特征的DNA弯曲区域的转录通读有关。这证明了RNA聚合酶II通过一种序列特异的DNA结合蛋白进行的因子依赖转录。新生的转录裂解可能是RNA聚合酶II绕过许多转录障碍的一般机制。
In eukaryotes the genetic material is contained within a coiled, protein-coated structure known as chromatin. RNA polymerases must recognize specific nucleoprotein assemblies and maintain contact with the underlying DNA duplex for many thousands of base pairs. Template-bound lac operon repressor from Escherichia coli arrests RNA polymerase II in vitro and in vivo [Kuhn, A., Bartsch, I. & Grummt, I. (1990) Nature (London) 344, 559-562; Deuschele, U., Hipskind, R. A. & Bujard, H. (1990) Science 248, 480-483]. We show that in a reconstituted transcription system, elongation factor SII enables RNA polymerase II to proceed through this blockage at high efficiency. lac repressor-arrested elongation complexes display an SII-activated transcript cleavage reaction, an activity associated with transcriptional read-through of a previously characterized region of bent DNA. This demonstrates factor-dependent transcription by RNA polymerase II through a sequence-specific DNA-binding protein. Nascent transcript cleavage may be a general mechanism by which RNA polymerase II can bypass many transcriptional impediments.