Toxicology Study of Single-walled Carbon Nanotubes and Reduced Graphene Oxide in Human Sperm.
Toxicology Study of Single-walled Carbon Nanotubes and Reduced Graphene Oxide in Human Sperm.
复制标题
DOI：
10.1038/srep30270
复制
发表时间：
2016-08-19
影响因子：
4.6
通讯作者：
Demirci U
中科院分区：
文献类型：
--
作者：
Asghar W;Shafiee H;Velasco V;Sah VR;Guo S;El Assal R;Inci F;Rajagopalan A;Jahangir M;Anchan RM;Mutter GL;Ozkan M;Ozkan CS;Demirci U
目前正在评估单壁碳纳米管和还原氧化石墨烯等碳基纳米材料的生物医学应用，包括体内药物输送和肿瘤成像。一些报告研究了碳纳米材料的毒性，但它们对人类男性生殖的影响尚未得到充分检验。此外，尚不清楚纳米材料暴露是否对临床环境中使用的精子分选程序有任何影响。在这里，我们表明，功能化单壁碳纳米管 (SWCNT-COOH) 和浓度为 1–25μg/mL 的还原氧化石墨烯的存在不会影响精子活力。然而，SWCNT-COOH 在较高浓度 (25μg/mL) 下会产生显着的反应性超氧化物物质，而还原的氧化石墨烯不会在人类精子中引发反应性物质。此外，我们证明暴露于这些纳米材料不会阻碍精子分选过程，并且微流体分选系统可以选择暴露后表现出低氧化应激的精子。
Carbon-based nanomaterials such as single-walled carbon nanotubes and reduced graphene oxide are currently being evaluated for biomedical applications including in vivo drug delivery and tumor imaging. Several reports have studied the toxicity of carbon nanomaterials, but their effects on human male reproduction have not been fully examined. Additionally, it is not clear whether the nanomaterial exposure has any effect on sperm sorting procedures used in clinical settings. Here, we show that the presence of functionalized single walled carbon nanotubes (SWCNT-COOH) and reduced graphene oxide at concentrations of 1–25 μg/mL do not affect sperm viability. However, SWCNT-COOH generate significant reactive superoxide species at a higher concentration (25 μg/mL), while reduced graphene oxide does not initiate reactive species in human sperm. Further, we demonstrate that exposure to these nanomaterials does not hinder the sperm sorting process, and microfluidic sorting systems can select the sperm that show low oxidative stress post-exposure.