N-acetylcysteine slows down ageing and increases the life span of Drosophila melanogaster
N-acetylcysteine slows down ageing and increases the life span of Drosophila melanogaster
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DOI：
10.1007/pl00013199
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发表时间：
1997-12-01
影响因子：
8
通讯作者：
Labuhn, M
中科院分区：
文献类型：
--
作者：
Brack, C;Bechter-Thüring, E;Labuhn, M
衰老可以被定义为生物体生理功能随时间的衰退。衰老过程的分子原因是多方面的，涉及遗传和环境因素。有人提出，抗氧化剂可能会积极影响衰老过程，保护生物体免受自由基引起的损害。在这里，我们减缓了抗氧化剂N-乙酰半胱氨酸（NAC）对黑腹果蝇具有延长寿命的作用。饮食摄取NAC导致中位寿命和最大寿命呈剂量依赖性增加。喂食1 mg/ml NAC食物的苍蝇寿命延长16.6%;在10 mg/ml时，寿命延长26.6%。我们已经检查了NAC治疗对蛋白质和RNA水平的影响：我们观察到总RNA和核糖体RNA的绝对量的NAC依赖性增加，但蛋白质水平没有差异。NAC对寿命的影响可能涉及特定mRNA基因的差异表达，如RNA指纹实验所示。
Ageing can be defined as the time-dependent decline of physiological functions of an organism. The molecular causes for the ageing process are multiple, involving both genetic and environmental factors. It has been proposed that antioxidants may positively influence the ageing process, protecting the organism against free radical-induced damage. Here we slow that the antioxidant N-acetylcysteine (NAC) has a life-extending effect on Drosophila melanogaster. Dietary uptake of NAC results in a dose-dependent increase in median and maximum life span. Flies fed on 1 mg/ml NAC food live 16.6%, longer; at 10 mg/ml, life span increases by 26.6%. We have examined the effect of NAC treatment on protein and RNA levels: we observe an NAC-dependent increase in absolute amounts of total RNA and ribosomal RNA, but no differences in protein levels. The NAC effect on longevity may involve differential expression of specific mRNA genes, as suggested by RNA finger-printing experiments.