The chromatin-remodeling factor AtINO80 plays crucial roles in genome stability maintenance and in plant development.
The chromatin-remodeling factor AtINO80 plays crucial roles in genome stability maintenance and in plant development.
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DOI：
10.1111/tpj.12840
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发表时间：
2015-05
期刊：
影响因子：
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通讯作者：
Chi Zhang;Lin Cao;L. Rong;Zengxuan An;Wangbin Zhou;Jinbiao Ma;W. Shen;Yan Zhu;A. Dong
中科院分区：
文献类型：
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作者：
Chi Zhang;Lin Cao;L. Rong;Zengxuan An;Wangbin Zhou;Jinbiao Ma;W. Shen;Yan Zhu;A. Dong
INO 80是真核生物中一个保守的染色质重塑因子。虽然先前的研究报道拟南芥INO 80（AtINO 80）是体细胞同源重组（HR）所必需的，但AtINO 80在植物生长和发育中的作用仍然不清楚。在这里，我们确定和表征两个独立的atino 80突变等位基因，atino 80 -5和atino 80 -6，显示相似的和多效性的表型，包括较小的植物和器官大小，和晚开花。在标准生长条件下，atino 80 -5显示HR降低;然而，经过遗传毒性处理后，突变体的HR增加，伴随着更多的DNA双链断裂和更强的细胞反应。转录分析表明，许多发育和环境响应基因在atino 80 -5中的扰动基因中过量表达。这些基因与H2A.Z体富集基因的类别显著重叠。AtINO 80还与H2A.Z相互作用，并促进H2A.Z在关键开花阻遏基因FLC和MAF 4/5末端的富集。我们对atino 80 -5和atino 80 -6突变体的表征证实并扩展了以前的AtINO 80研究，并为将植物染色质稳定性中涉及的表观遗传机制与植物对发育和环境线索的反应联系起来的研究提供了前景。
INO80 is a conserved chromatin-remodeling factor in eukaryotes. While a previous study reported that the Arabidopsis thaliana INO80 (AtINO80) is required for somatic homologous recombination (HR), the role of AtINO80 in plant growth and development remains obscure. Here, we identified and characterized two independent atino80 mutant alleles, atino80-5 and atino80-6, which display similar and pleiotropic phenotypes, including smaller plant and organ size, and late flowering. Under standard growth conditions, atino80-5 showed decreased HR; however, after genotoxic treatment, HR in the mutant increased, accompanied by more DNA double-strand breaks and stronger cellular responses. Transcription analysis showed that many developmental and environmental responsive genes are overrepresented in the perturbed genes in atino80-5. These genes significantly overlapped with the category of H2A.Z body-enriched genes. AtINO80 also interacts with H2A.Z, and facilitates the enrichment of H2A.Z at the ends of the key flowering repressor genes FLC and MAF4/5. Our characterization of the atino80-5 and atino80-6 mutants confirms and extends the previous AtINO80 study, and provides perspectives for linking studies of epigenetic mechanisms involved in plant chromatin stability with plant response to developmental and environmental cues.