Comparison of wild-type and subacute sclerosing panencephalitis strains of measles virus. Neurovirulence in ferrets and biological properties in cell cultures.
Comparison of wild-type and subacute sclerosing panencephalitis strains of measles virus. Neurovirulence in ferrets and biological properties in cell cultures.
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DOI：
10.1084/jem.148.3.674
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发表时间：
1978-09-01
期刊：
影响因子：
--
通讯作者：
Brown HR
中科院分区：
文献类型：
--
作者：
Thormar H;Mehta PD;Brown HR
通过脑内接种受感染的Vero细胞悬液，研究了2种野生型（wt）和7种亚急性硬化性全脑炎（SSPE）麻疹病毒株在年轻成年雪貂中的神经毒力。Wt菌株Edmonston和Woodfolk以及SSPE菌株Mantooth、Halle和LEC-S在雪貂中未产生可检测到的脑炎，但引起血清中明显形成针对麻疹病毒的抗体。另一方面，SSPE菌株LEC、IP-3、Biken和D.R在雪貂中均具有神经毒性，特别是菌株D.R在所有接种动物中均引起急性脑炎。Biken毒株引起了特别的兴趣，因为它在7只雪貂中引起了4只的亚急性脑炎。亚急性脑炎的特点是潜伏期长，病毒在大脑中持续存在至少8个月，广泛的炎症病变，并在大脑中产生麻疹病毒特异性IgG。对各种麻疹病毒株生物学特性的研究表明，wt株Edmonston和Woodfolk以及SSPE株Mantooth、Halle和LEC-S在Vero和雪貂脑（FB）培养物中均产生显著滴度的游离病毒颗粒。在Vero培养中，细胞病变效应（CPE）与细胞融合显著，而在FB培养中，仅观察到最小的CPE和细胞融合。另一方面，SSPE菌株LEC、IP-3、Biken和D.R在Vero和FB培养中主要与细胞相关，尽管菌株Biken和IP-3产生非典型无细胞颗粒。所有四种菌株在FB培养中都表现出细胞融合活性，特别是菌株D.R，它是唯一一种在FB中比在Vero培养中更活跃地融合传播的菌株。结果讨论了各种麻疹病毒株在成年雪貂神经毒力的关系。FB细胞中明显的细胞融合活性和细胞结合与很少或不产生无细胞病毒似乎是在动物中建立脑感染所必需的。
The neurovirulence of two wild type (wt) and seven Subacute Sclerosing Panencephalitis (SSPE) measles virus strains was tested in young adult ferrets by intracerebral (IC) inoculation of infected Vero cell suspensions. Wt strains Edmonston and Woodfolk and SSPE strains Mantooth, Halle, and LEC-S did not produce a detectable encephalitis in the ferrets, but caused a significant formation of serum antibodies against measles virus. SSPE strains LEC, IP-3, Biken, and D.R., on the other hand, were all neurovirulent in ferrets, particularly strain D.R. which caused an acute encephalitis in all inoculated animals. Strain Biken was of particular interest since it caused a subacute encephalitis in four of seven ferrets. The subacute encephalitis was characterized by a long incubation time, persistence of virus in the brain for at least 8 mo, widespread inflammatory lesions, and production of measles virus specific IgG in the brain. A study of the biological properties of the various measles virus strains showed that wt strains Edmonston and Woodfolk and SSPE strains Mantooth, Halle, and LEC-S produced free virus particles in significant titers both in Vero and ferret brain (FB) cultures. Cytopathic effect (CPE) with cell- fusion was marked in Vero cultures, whereas only minimal CPE and no cell-fusion were observed in the FB cultures. SSPE strains LEC, IP-3, Biken, and D.R., on the other hand, were mostly cell-associated in Vero and FB cultures, although atypical cell-free particles were produced by strains Biken and IP-3. All four strains showed cell-fusing activity in FB cultures, particularly strain D.R., which was the only strain that spread more actively by fusion in FB than in Vero cultures. The results are discussed in relation to the neurovirulence of the various measles virus strains in adult ferrets. Pronounced cell-fusing activity in FB cells and cell-association with minimal or no production of cell-free virus seem to be essential to establish a brain infection in the animals.