Isolation of an (O,H,Vi)-free immunoprotective antigenic fraction with mannose receptor-like activity from Salmonella typhi.
Isolation of an (O,H,Vi)-free immunoprotective antigenic fraction with mannose receptor-like activity from Salmonella typhi.
复制标题
从伤寒沙门氏菌中分离出具有甘露糖受体样活性的不含 (O,H,Vi) 的免疫保护性抗原部分。
DOI：
10.1093/infdis/143.1.106
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
J. Alouf
中科院分区：
文献类型：
--
作者：
H. Jouin;A. Staub;J. Alouf
伤寒沙门氏菌TY6S株的抗原组分，通过1.5M氯化钠洗涤和5%-20%蔗糖密度梯度超速离心法从细菌颗粒中获得，可诱导兔血清抗体，保护用致死剂量的伤寒沙门氏菌攻击的鸡胚。该抗原制剂不含Vi、O和H抗原。它含有约85%的蛋白质和Rd1P+脂多糖。蛋白质部分由三个多肽组成，分别为45,000，32,000和20,000道尔顿。此外，纯化的制剂表现出细菌粘附素的性质，对甘露醇残基具有受体样的活性，通过在其表面凝集含有这些残基的靶细胞来表明。
An antigenic fraction of Salmonella typhi strain TY6S, obtained from a bacterial pellet by washings with 1.5 M NaCl and ultracentrifugation in a 5%-20% sucrose density gradient, induced serum antibodies in rabbits that protected chicken embryos challenged with lethal doses of the virulent microorganism. The antigenic preparation was free of Vi, O, and H antigens. It contained about 85% protein and Rd1P+ lipopolysaccharide. The protein moiety comprised three polypeptides of 45,000, 32,000, and 20,000 daltons, respectively. In addition, the purified preparation exhibited the properties of a bacterial adhesin with receptor-like activity for mannosyl residues, as shown by agglutination of target cells containing these residues at their surfaces.