Microbial community analysis of deeply buried marine sediments of the New Jersey shallow shelf (IODP Expedition 313)
Microbial community analysis of deeply buried marine sediments of the New Jersey shallow shelf (IODP Expedition 313)
复制标题
DOI：
10.1111/1574-6941.12146
复制
发表时间：
2013-09-01
影响因子：
4.2
通讯作者：
Schippers, Axel
中科院分区：
文献类型：
--
作者：
Breuker, Anja;Stadler, Susanne;Schippers, Axel
所研究的美国新泽西州浅海陆架深埋海洋沉积物的特点是有机碳含量低，每毫升沉积物中的细胞总数< 10(7) 个。细菌和古细菌的 qPCR 数据与总细胞计数处于同一数量级。古细菌和细菌在沉积物上部的 16S rRNA 基因拷贝数相似，但细菌在分析的沉积物岩心的最下部占主导地位，最大分析深度为 c。海底以下 50 米 (mbsf)。候选细菌门 JS1 以及 Chloroflexi 的 Anaerolineae 和 Caldinilineae 几乎与细菌总数一样丰富。硫酸盐还原剂也发现了同样高的 dsrA 基因拷贝数。上层 c 中包含地杆菌科的 Fe(III) 和 Mn(IV) 还原剂的丰度。 15 mbsf 与孔隙水中锰和铁的浓度相关。克隆文库中分离的古菌16S rRNA基因序列可分别以1%、14%和85%的比例归属于奇古菌门、广古菌门和泉古菌门。沉积物群落中典型的深层地下沉积物相关类群MBG-B、MBG-D、MCG 和SAGMEG 均有代表。 MCG 是分离的 16S rRNA 基因序列具有高度多样性的优势群体。
The investigated deeply buried marine sediments of the shallow shelf off New Jersey, USA, are characterized by low organic carbon content and total cell counts of < 10(7) cells per mL sediment. The qPCR data for Bacteria and Archaea were in the same orders of magnitude as the total cell counts. Archaea and Bacteria occurred in similar 16S rRNA gene copy numbers in the upper part of the sediments, but Bacteria dominated in the lowermost part of the analyzed sediment cores down to a maximum analyzed depth of c. 50 meters below seafloor (mbsf). The bacterial candidate division JS1 and the classes Anaerolineae and Caldinilineae of the Chloroflexi were almost as highly abundant as the total Bacteria. Similarly high dsrA gene copy numbers were found for sulfate reducers. The abundance of the Fe(III) and Mn(IV) reducers comprising Geobacteraceae in the upper c. 15 mbsf correlated with concentrations of manganese and iron in the pore water. The isolated 16S rRNA gene sequences of Archaea in clone libraries could be allocated to the phyla Thaumarchaeota, Euryarchaeota, and Crenarchaeota with 1%, 14%, and 85%, respectively. The typical deep subsurface sediment-associated groups MBG-B, MBG-D, MCG, and SAGMEG were represented in the sediment community. MCG was the dominant group with a high diversity of the isolated 16S rRNA gene sequences.