IL-27 Modulates Chemokine Production in TNF-α-Stimulated Human Oral Epithelial Cells
IL-27 Modulates Chemokine Production in TNF-α-Stimulated Human Oral Epithelial Cells
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DOI：
10.1159/000481760
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发表时间：
2017-01-01
影响因子：
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通讯作者：
Matsuo, Takashi
中科院分区：
文献类型：
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作者：
Hosokawa, Yoshitaka;Hosokawa, Ikuko;Matsuo, Takashi
白细胞介素-27（IL-27）是IL-12家族的一种细胞因子。然而，IL-27在牙周病发病机制中的作用尚不确定。本研究的目的是检测IL-27对TNF-α刺激的人口腔上皮细胞（TR 146）中趋化因子产生的影响。方法：采用ELISA法检测TR 146细胞趋化因子的产生。我们使用western blot分析来检测TR 146中的信号转导分子，包括STAT 1和STAT 3的磷酸化水平。我们使用抑制剂来研究STAT 1和STAT 3激活的作用。结果：IL-27增加TNF-α刺激的TR 146中CXCR 3配体的产生。同时，IL-27抑制TNF-α诱导的IL-8和CCL 20的产生。与TNF-α刺激的TR 146相比，IL-27和TNF-α刺激的TR 146中的STAT 1磷酸化水平增强。IL-27处理的TR 146中的STAT 3磷酸化水平并未因TNF-α而改变。STAT 1抑制剂和STAT 3抑制剂均降低CXCR 3配体的产生。在TNF-α刺激的TR 146中，STAT 1抑制剂超过了IL-27对IL-8和CCL 20产生的抑制作用。同时，STAT 3抑制剂不调节IL-8和CCL 20的产生。结论：IL-27可能通过调节牙周组织中上皮细胞趋化因子的产生来调控白细胞的迁移。(C)2017作者（s）由S. Karger AG，巴塞尔
Background/Aims: Interleukin-27 (IL-27) is a cytokine which belongs to the IL-12 family. However, the role of IL-27 in the pathogenesis of periodontal disease is uncertain. The aim of this study was to examine the effect of IL-27 on chemokine production in TNF-alpha-stimulated human oral epithelial cells (TR146). Methods: We measured chemokine production in TR146 by ELISA. We used western blot analysis to detect the phosphorylation levels of signal transduction molecules, including STAT1 and STAT3 in TR146. We used inhibitors to examine the role of STAT1 and STAT3 activation. Results: IL-27 increased CXCR3 ligands production in TNF-alpha-stimulated TR146. Meanwhile, IL-27 suppressed IL-8 and CCL20 production induced by TNF-alpha. STAT1 phosphorylation level in IL-27 and TNF-alpha-stimulated TR146 was enhanced in comparison to TNF-alpha-stimulated TR146. STAT3 phosphorylation level in IL-27-treated TR146 did not change by TNF-alpha. Both STAT1 inhibitor and STAT3 inhibitor decreased CXCR3 ligands production. STAT1 inhibitor overrode the inhibitory effect of IL-27 on IL-8 and CCL20 production in TNF-alpha-stimulated TR146. Meanwhile, STAT3 inhibitor did not modulate IL-8 and CCL20 production. Conclusion: IL-27 might control leukocyte migration in periodontal lesion by modulating chemokine production from epithelial cells. (C) 2017 The Author(s) Published by S. Karger AG, Basel