New hepatitis C virus (HCV) genotyping system that allows for identification of HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5a, and 6a
New hepatitis C virus (HCV) genotyping system that allows for identification of HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5a, and 6a
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DOI：
10.1128/jcm.35.1.201-207.1997
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发表时间：
1997-01-01
影响因子：
9.4
通讯作者：
Lau, JYN
中科院分区：
文献类型：
--
作者：
Ohno, T;Mizokami, M;Lau, JYN
最近的研究集中在不同的丙型肝炎病毒（HCV）基因型是否与不同的致病性、感染性和抗病毒治疗反应有关。建立一种简单、准确的HCV基因分型方法是解决上述问题的关键。本研究建立了一种基于核心区PCR的HCV基因分型方法，并对607份标本进行了HCV基因型1a、1b、2a、2b、3a、3b、4、5a、6a的检测（379份来自日本，63份来自美国，53份来自韩国，35份来自台湾，32份来自中国，20份来自香港，15份来自澳大利亚，6份来自埃及，3份来自孟加拉国，1份来自南非）通过Okamoto等人（H.冈本岛，澳-地Sugiyama，S. Okada，K. Kurai，Y. Akahane，Y. Sugai，T.田中角佐藤角，澳-地Tsuda，Y. Miyamura和M. Mayumi，J. Gen. Virol. 73：673-679，1992）和这种新的基因分型系统。结果比较显示，539份样品（88.8%）的结果一致。在68例不一致的标本中，对23例标本进行了核苷酸序列测定，并对其进行了基因型的分子进化分析。该基因分型系统可用于临床研究中HCV基因型的大规模测定。
Recent studies have focused on whether different hepatitis C virus (HCV) genotypes are associated,vith different profiles of pathogenicity, infectivity, and response to antiviral therapy. The establishment of a simple and precise genotyping system for HCV is essential to address these issues, A new genotyping system based on PCR of the core region with genotype-specific PCR primers for the determination of HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5a, and 6a was developed, A total of 607 samples (379 from Japan, 63 from the United States, 53 from Korea, 35 from Taiwan, 32 from China, 20 from Hong Kong, 15 from Australia, 6 from Egypt, 3 from Bangladesh, and 1 from South Africa) were tested by both the assay of Okamoto et al. (H. Okamoto, Y. Sugiyama, S. Okada, K. Kurai, Y. Akahane, Y. Sugai, T. Tanaka, K. Sato, F. Tsuda, Y. Miyamura, and M. Mayumi, J. Gen. Virol. 73:673-679, 1992) and this new genotyping system. Comparison of the results showed concordant results for 539 samples (88.8%). Of the 68 samples with discordant results, the nucleotide sequences of the HCV isolates were determined in 23, and their genotypes were determined by molecular evolutionary analysis, In all 23 samples, the assignment of genotype by our new genotyping system was correct. This genotyping system may be useful for large-scale determination of HCV genotypes in clinical studies.