High levels of Cellular Prion Protein improve astrocyte development
High levels of Cellular Prion Protein improve astrocyte development
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DOI：
10.1016/j.febslet.2012.11.032
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发表时间：
2013-01-16
期刊：
影响因子：
3.5
通讯作者：
Souza Lima, Flavia Regina
中科院分区：
文献类型：
--
作者：
Hartmann, Camila Arantes;Martins, Vilma Regina;Souza Lima, Flavia Regina
朊病毒蛋白（PrPC）具有神经保护功能，在这里，我们证明，从PrPC过表达小鼠的星形胶质细胞比野生型星形胶质细胞更耐诱导细胞死亡。应激诱导蛋白1（STI 1）是一种PrPC配体，通过激活蛋白激酶A防止野生型和PrPC过表达星形胶质细胞的细胞死亡。培养的胚胎星形胶质细胞和脑提取物从PrPC过度表达小鼠显示较高的胶质细胞酸性蛋白表达和减少波形蛋白和巢蛋白水平相比，野生型星形胶质细胞，表明更快的星形胶质细胞成熟在前小鼠。我们的数据表明，PrPC水平调节星形胶质细胞的发育，PrPC-STI 1相互作用有助于防止星形胶质细胞死亡。(C)2012年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
Prion protein (PrPC) has neuroprotective functions and herein we demonstrate that astrocytes from PrPC-over-expressing mice are more resistant to induced cell death than wild-type astrocytes. The Stress-Inducible-Protein 1 (STI1), a PrPC ligand, prevents cell death in both wild-type and PrPC-over-expressing astrocytes through the activation of protein-kinase-A. Cultured embryonic astrocytes and brain extracts from PrPC-over-expressing mice show higher glial fibrillary acidic protein expression and reduced vimentin and nestin levels when compared to wild-type astrocytes, suggesting faster astrocyte maturation in the former mice. Our data indicate that PrPC levels modulate astrocyte development, and that PrPC-STI1 interaction contributes to protect against astrocyte death. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.