LYSOGENY AND BACTERIOCINOGENY IN XENORHABDUS-NEMATOPHILUS AND OTHER XENORHABDUS SPP
LYSOGENY AND BACTERIOCINOGENY IN XENORHABDUS-NEMATOPHILUS AND OTHER XENORHABDUS SPP
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DOI：
10.1128/aem.58.9.3032-3037.1992
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发表时间：
1992-09-01
影响因子：
4.4
通讯作者：
BREHELIN, M
中科院分区：
文献类型：
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作者：
BOEMARE, NE;BOYERGIGLIO, MH;BREHELIN, M
经丝裂霉素或高温处理后，嗜线虫Xnowhabdus nematophilus A24两相均产生细菌素和噬菌体，表明溶原性。从嗜线虫A24中提纯的噬菌体DNA与消化后的A24染色体DNA片段杂交，证实噬菌体基因组已整合到细菌染色体中。细菌素和噬菌体在大多数其他致病杆菌的培养中都能被检测到。经丝裂霉素或高温处理后。这些处理不能裂解发光杆菌K80，也没有发现与该菌株相关的噬菌体。然而，细菌素在包括发光菌K80在内的所有致病杆菌培养物中都检测到了有限的量，没有任何诱导。嗜线虫A24细菌素对其他菌种有拮抗作用，但对A24或其他嗜线虫菌株无拮抗作用。
Induction by mitomycin or high-temperature treatment resulted in the production of bacteriocins and phages in both phases of Xenorhabdus nematophilus A24, indicating lysogeny. Phage DNA purified from X. nematophilus A24 hybridized to several fragments of DraI-digested A24 chromosomal DNA, confirming that the phage genome was incorporated into the bacterial chromosome. Bacteriocins and phages were detected in cultures of most other Xenorhabdus spp. after mitomycin or high-temperature treatment. Xenorhabdus luminescens K80 was not lysed by these treatments, and no phages were seen associated with this strain. However, bacteriocins were detected in limited quantities in all Xenorhabdus cultures, including X. luminescens K80, without any induction. X. nematophilus A24 bacteriocins were antagonistic for other Xenorhabdus species but not for A24 or other strains of X. nematophilus.