Identification of SACE_7040, a Member of TetR Family Related to the Morphological Differentiation of Saccharopolyspora erythraea
Identification of SACE_7040, a Member of TetR Family Related to the Morphological Differentiation of Saccharopolyspora erythraea
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红糖多孢菌形态分化相关TetR家族成员SACE_7040的鉴定
DOI：
10.1007/s00284-011-9943-z
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发表时间：
2011-08-01
影响因子：
2.6
通讯作者：
Zhang, Buchang
中科院分区：
文献类型：
--
作者：
Han, Shu;Song, Ping;Zhang, Buchang
SACE_7040被认为是TetR家族的成员，转录调节子在多孢霉，但其生物学功能是未知的。结果表明，SACE_7040基因敲除突变体比原始菌株更早形成气生菌丝，并且通过SACE_7040基因的单拷贝互补可以恢复气生菌丝的表型，表明SACE_7040基因是Sac形态分化的重要调控因子。菜当SACE_7040基因在bldD突变体中被破坏时，我们有趣地发现，bldD突变体所表现出的气生发育缺陷可以被克服，这表明SACE_7040和BldD在Sac中之间存在串扰。菜形态发生这些发现提供了新的见解对囊。菜发育生物学
SACE_7040 is presumed to be a member of the TetR family of transcriptional regulators in Saccharopolyspora erythraea, but its biological function is unknown. It was shown that the SACE_7040 gene knockout mutant formed aerial mycelium earlier than its original strain, and this phenotype could be restored by complementation of a single copy of SACE_7040 gene, demonstrating that SACE_7040 is an important regulator of the morphological differentiation of Sac. erythraea. When SACE_7040 gene was disrupted in the bldD mutant, we intriguingly found that the defect in aerial development exhibited by the bldD mutant could be overcome, suggesting a crosstalk between SACE_7040 and BldD in Sac. erythraea morphogenesis. These findings provide novel insights toward the Sac. erythraea developmental biology.