Self-assembled combinatorial encoding nanoarrays for multiplexed biosensing
Self-assembled combinatorial encoding nanoarrays for multiplexed biosensing
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DOI：
10.1021/nl062998n
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发表时间：
2007-02-01
期刊：
影响因子：
10.8
通讯作者：
Yan, Hao
中科院分区：
文献类型：
--
作者：
Lin, Chenxiang;Liu, Yan;Yan, Hao
在生物标志物分析和疾病诊断中，对来自单一溶液和少量样品的核酸、蛋白质或其他分子的多重且灵敏的检测是非常需要的。在这里，我们描述了一个新的概念，使用组合自组装的DNA纳米片到微米大小的二维阵列，携带核酸探针和条形码荧光染料，以实现多重检测。我们通过检测多个DNA序列和适体结合分子证明了阵列的特异性和灵敏度。这种基于DNA瓦片阵列的传感器平台可以通过DNA自组装来构建。不同分子探针的连接可以通过简单的DNA杂交来实现，因此生物缀合对于标记是不必要的。探针间距离和基于溶液的结合反应的精确控制确保了快速的靶结合动力学。
Multiplexed and sensitive detection of nucleic acids, proteins, or other molecules from a single solution and a small amount of sample is of great demand in biomarker profiling and disease diagnostics. Here we describe a new concept using combinatorial self-assembly of DNA nanotiles into micrometer-sized two-dimensional arrays that carry nucleic acid probes and barcoded fluorescent dyes to achieve multiplexed detection. We demonstrated the specificity and sensitivity of the arrays by detecting multiple DNA sequences and aptamer binding molecules. This DNA tile-array-based sensor platform can be constructed through DNA self-assembly. The attachment of different molecular probes can be achieved by simple DNA hybridization so bioconjugation is not necessary for the labeling. Accurate control of the interprobe distances and solution-based binding reactions ensures fast target binding kinetics.