Expression of epidermal growth factor, transforming growth factor-alpha and their receptor genes in human gastric carcinomas; implication for autocrine growth.
Expression of epidermal growth factor, transforming growth factor-alpha and their receptor genes in human gastric carcinomas; implication for autocrine growth.
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DOI：
10.1111/j.1349-7006.1990.tb02505.x
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发表时间：
1990-01
期刊：
影响因子：
--
通讯作者：
Tahara E
中科院分区：
文献类型：
--
作者：
Yoshida K;Kyo E;Tsujino T;Sano T;Niimoto M;Tahara E
检测了7株人胃癌细胞系、15例胃癌组织及相应正常粘膜中表皮生长因子（EGF）、转化生长因子-α（TGF-α）和表皮生长因子受体（EGFR）基因mRNA的表达。所有胃癌细胞系均表达EGFR和TGF-α基因的mRNA。TMK-1和MKN-28细胞也表达EGF mRNA。EGF和TGF-α特异性单克隆抗体结合也证实了胃癌细胞系产生EGF、TGF-α和EGFR蛋白。对于手术标本，EGFR和TGF-α mRNA在所有肿瘤组织中均以高水平检测到。15例胃癌组织中有5例（33.3%）表达EGFmRNA，而正常胃组织中未见表达。此外，抗EGF和抗TGF-α单克隆抗体抑制胃癌细胞自发摄取3 H-TdR。这些结果表明，肿瘤细胞产生的EGF和/或TGF-α作为胃癌的自分泌生长因子。
The expressions of mRNA for epidermal growth factor (EGF), transforming growth factor‐α (TGF‐α) and EGF receptor (EGFR) genes were examined in 7 human gastric carcinoma cell lines and 15 gastric carcinoma tissues and the corresponding normal mucosas. All of the gastric carcinoma cell lines expressed mRNA for EGFR and TGF‐α genes. TMK‐1 and MKN‐28 cells also expressed EGF mRNA. Production of EGF, TGF‐α and EGFR protein by gastric carcinoma cell lines was also confirmed by EGF and TGF‐α specific monoclonal antibody binding. As for surgical specimens, EGFR and TGF‐α mRNA were detected at high levels in all the tumor tissues. Interestingly, EGF mRNA was detected in 5 (33.3%) of the 15 gastric carcinomas but it was not detected in normal tissues. Moreover, anti‐EGF and anti‐TGF‐α monoclonal antibodies inhibited the spontaneous 3H‐TdR uptake by gastric carcinoma cells. These results suggest that EGF and/or TGF‐α produced by tumor cells act as autocrine growth factors for gastric carcinomas.