A nuclear gene in maize required for the translation of the chloroplast atpB/E mRNA
A nuclear gene in maize required for the translation of the chloroplast atpB/E mRNA
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DOI：
10.1105/tpc.11.9.1709
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发表时间：
1999-09-01
期刊：
影响因子：
11.6
通讯作者：
Barkan, A
中科院分区：
文献类型：
--
作者：
McCormac, DJ;Barkan, A
为了阐明调控陆地植物叶绿体翻译的机制，我们在玉米中寻找破坏叶绿体mrna亚群翻译的核突变。证据提出了一个核基因，其功能是翻译叶绿体atpB/E mRNA所必需的。由于atp1亚基的合成减少，atp1的突变导致叶绿体ATP合成酶复合物的积累失败。AtpB合成的减少不是由于AtpB mRNA结构或丰度的改变。相反，atpbmrna与atp1-1突变体中异常少的核糖体相关，表明在翻译起始或延伸早期需要atp1功能。在此之前，只在陆地植物中发现了一个翻译特定叶绿体mrna所需的核基因。因此，atp1将成为分析叶绿体中翻译控制机制的有用工具。
To elucidate mechanisms that regulate chloroplast translation in land plants, we sought nuclear mutations in maize that disrupt the translation of subsets of chloroplast mRNAs. Evidence is presented for a nuclear gene whose function is required for the translation of the chloroplast atpB/E mRNA. A mutation in atp1 results in a failure to accumulate the chloroplast ATP synthase complex due to reduced synthesis of the AtpB subunit. This decrease in AtpB synthesis does not result from a change in atpB mRNA structure or abundance. Instead, the atpB mRNA is associated with abnormally few ribosomes in atp1-1 mutants, indicating that atp1 function is required during translation initiation or early in elongation. Previously, only one nuclear gene that is required for the translation of specific chloroplast mRNAs had been identified in a land plant. Thus, atp1 will be a useful tool for dissecting mechanisms of translational control in chloroplasts.