The Complete Mitochondrial Genome Sequence of Hepatozoon catesbianae (Apicomplexa: Coccidia: Adeleorina), a Blood Parasite of the Green Frog, Lithobates (Formerly Rana) clamitans

The Complete Mitochondrial Genome Sequence of Hepatozoon catesbianae (Apicomplexa: Coccidia: Adeleorina), a Blood Parasite of the Green Frog, Lithobates (Formerly Rana) clamitans
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绿蛙、Lithobates(以前的林蛙)clamitans 的血液寄生虫 Hepatozoon catesbianae(顶端复门:球虫:Adeleorina)的完整线粒体基因组序列

DOI:
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发表时间:
2014
影响因子:
1.3
通讯作者:
John R Barta
John R Barta
中科院分区:
医学4区
文献类型:
--
作者:
Alexandre N. Léveillé;M. E. Ogedengbe;M. Hafeez;Hsiang;John R Barta

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摘要/ Abstract摘要:对血寄生虫catesbianae Hepatozoon (Alveolata; apiccomplex A; Coccidia; Adeleorina; Hepatozoidae)的线粒体基因组进行PCR扩增,并对PCR产物进行直接测序。H. catesbianae线粒体基因组长度为6397 bp,包含3个蛋白质编码基因(细胞色素c氧化酶亚基I [COI]、细胞色素c氧化酶亚基III [COIII]和细胞色素B [CytB])。与先前发表的其他顶复合体寄生虫线粒体基因组序列的相似性允许在H. catesbianae线粒体基因组中注释23个假定的rDNA片段,14个大亚基rDNA片段和9个小亚基rDNA片段。恶性疟原虫rDNA片段RNA5、RNA8、RNA11和RNA19的对应序列未在猫疟原虫线粒体基因组序列中发现。尽管存在3个蛋白质编码区和许多推测的rDNA片段是顶复合体线粒体基因组的典型特征,但H. catesbianae的线粒体基因组具有与顶复合体不同的结构和基因组织。这是第一次从尖复合体亚目的寄生虫中获得完整的线粒体基因组序列。
Abstract: A complete mitochondrial genome for the blood parasite Hepatozoon catesbianae (Alveolata; Apicomplexa; Coccidia; Adeleorina; Hepatozoidae) was obtained through PCR amplification and direct sequencing of resulting PCR products. The mitochondrial genome of H. catesbianae is 6,397 bp in length and contains 3 protein-coding genes (cytochrome c oxidase subunit I [COI]; cytochrome c oxidase subunit III [COIII]; and cytochrome B [CytB]). Sequence similarities to previously published mitochondrial genomes of other apicomplexan parasites permitted annotation of 23 putative rDNA fragments in the mitochondrial genome of H. catesbianae, 14 large subunit rDNA fragments, and 9 small subunit rDNA fragments. Sequences corresponding to rDNA fragments RNA5, RNA8, RNA11, and RNA19 of Plasmodium falciparum were not identified in the mitrochondrial genome sequence of H. catesbianae. Although the presence of 3 protein-coding regions and numerous putative rDNA fragments is a feature typical for apicomplexan mitochondrial genomes, the mitochondrial genome of H. catesbianae possesses a structure and gene organization that is distinct among the Apicomplexa. This is the first complete mitochondrial genome sequence obtained from any apicomplexan parasite in the suborder Adeleorina.
DOI: 10.1371/journal.pbio.1001419
发表时间: 2012
期刊: PLoS biology
影响因子: 9.8
作者:
Pawlowski J;Audic S;Adl S;Bass D;Belbahri L;Berney C;Bowser SS;Cepicka I;Decelle J;Dunthorn M;Fiore-Donno AM;Gile GH;Holzmann M;Jahn R;Jirků M;Keeling PJ;Kostka M;Kudryavtsev A;Lara E;Lukeš J;Mann DG;Mitchell EA;Nitsche F;Romeralo M;Saunders GW;Simpson AG;Smirnov AV;Spouge JL;Stern RF;Stoeck T;Zimmermann J;Schindel D;de Vargas C
通讯作者: de Vargas C