The dynein light chain Tctex-1 has a dynein-independent role in actin remodeling during neurite outgrowth
The dynein light chain Tctex-1 has a dynein-independent role in actin remodeling during neurite outgrowth
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DOI：
10.1016/j.devcel.2005.04.003
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发表时间：
2005-07-01
影响因子：
11.8
通讯作者：
Sung, CH
中科院分区：
文献类型：
--
作者：
Chuang, JZ;Yeh, TY;Sung, CH
协调的微管和微丝变化对于神经元的形态发育至关重要;然而，人们对连接这两个细胞骨架系统的潜在分子机制知之甚少。类似地，Rho-GTdR家族蛋白的不可或缺的作用已被证明，但尚不清楚它们的活性如何在不同的神经突中特异性地调节。在本文中，我们表明，细胞质动力蛋白轻链Tctex-1在海马神经元发育的多个步骤中起着关键作用，包括最初的神经突发芽，轴突规格，后来的树突的阐述。由Tctex-1引起的促神经突作用独立于其用于动力蛋白马达运输的货物适配器作用。最后，我们的数据表明，选择性高水平的Tctex-1在生长的轴突生长锥驱动快速轴突延伸，通过调节肌动蛋白动力学和Rac 1活性。
Coordinated microtubule and microfilament changes are essential for the morphological development of neurons; however, little is know about the underlying molecular machinery linking these two cytoskeletal systems. Similarly, the indispensable role of Rho-GTPase family proteins has been demonstrated, but it is unknown how their activities are specifically regulated in different neurites. In this paper, we show that the cytoplasmic dynein light chain Tctex-1 plays a key role in multiple steps of hippocampal neuron development, including initial neurite sprouting, axon specification, and later dendritic elaboration. The neuritogenic effects elicited by Tctex-1 are independent from its cargo adaptor role for dynein motor transport. Finally, our data suggest that the selective high level of Tctex-1 at the growth cone of growing axons drives fast neurite extension by modulating actin dynamics and also Rac1 activity.